Fytagoras B.V., Sylviusweg 72, 2333 BE Leiden, The Netherlands.
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650000, China.
Molecules. 2023 Jan 17;28(3):944. doi: 10.3390/molecules28030944.
Tyrosinase (TYR) plays a key role in the enzymatic reaction that is responsible for a range of unwanted discoloration effects, such as food browning and skin hyperpigmentation. TYR inhibitors could, therefore, be candidates for skin care products that aim to repair pigmentation problems. In this study, we used a metabolomics approach combined with the isobologram analysis to identify anti-TYR compounds within natural resources, and evaluate their possible synergism with each other. was determined to be a model plant for observing the effect, of which seven extracts with diverse phytochemicals were prepared by way of pressurized solvent extraction. Each extract (RPE) was profiled using nuclear magnetic resonance spectroscopy and its activity of tyrosinase inhibition was evaluated. According to the orthogonal partial least square analysis used to correlate phytochemicals in RPE with the corresponding activity, the goodness of fit of the model (R = 0.838) and its predictive ability (Q = 0.711) were high. Gallic acid and catechin were identified as the active compounds most relevant to the anti-TYR effect of RPE. Subsequently, the activity of gallic acid and catechin were evaluated individually, and when combined in various ratios by using isobologram analysis. The results showed that gallic acid and catechin in the molar ratios of 9:5 and 9:1 exhibited a synergistic inhibition on TYR, with a combination index lower than 0.77, suggesting that certain combinations of these compounds may prove effective for use in cosmetic, pharmaceutical, and food industries.
酪氨酸酶（TYR）在一系列非期望的变色反应中发挥关键作用，如食物褐变和皮肤色素沉着过度。因此，TYR 抑制剂可以成为旨在修复色素沉着问题的护肤品的候选物。在这项研究中，我们使用代谢组学方法结合等效应线分析来鉴定天然资源中的抗 TYR 化合物，并评估它们之间可能的协同作用。 被确定为观察效果的模式植物，其中七种具有不同植物化学物质的提取物通过加压溶剂提取法制备。使用核磁共振波谱法对每种 提取物（RPE）进行了分析，并评估了其对酪氨酸酶抑制的活性。根据正交偏最小二乘法分析，将 RPE 中的植物化学物质与相应的活性相关联，模型的拟合优度（R = 0.838）和预测能力（Q = 0.711）都很高。没食子酸和儿茶素被鉴定为与 RPE 的抗 TYR 作用最相关的活性化合物。随后，分别评估了没食子酸和儿茶素的活性，并通过等效应线分析以不同比例组合。结果表明，没食子酸和儿茶素在摩尔比为 9:5 和 9:1 时对 TYR 表现出协同抑制作用，组合指数低于 0.77，表明这些化合物的某些组合可能在化妆品、制药和食品工业中具有应用潜力。
