Hubbard N E, Erickson K L
Department of Human Anatomy, University of California, School of Medicine, Davis 95616.
Cancer Res. 1987 Dec 1;47(23):6171-5.
The influence of quantitative differences in dietary linoleic acid (18:2) on the metastasis as well as the development of line 4526 mouse mammary tumors was investigated. High fat diets (20%, w/w) that contained either 1, 2, 4, 8, or 12% 18:2 by weight, were prepared by using mixtures of coconut and safflower oil and fed to female BALB/c mice that were subsequently inoculated with 10(4) 4526 tumor cells s.c., either at the lateral abdominal wall (LAW) or in the mammary fat pad (MFP). Latency of LAW tumors was influenced by the level of dietary 18:2, whereas the latency of MFP tumors was not. When metastasis was assessed, mice with MFP tumors fed 1, 2, 4, or 8% 18:2 diets had 62-73% fewer lung surface tumor nodules than similar mice fed 12% 18:2. Mice in all dietary groups with LAW tumors had fewer metastatic lung nodules than mice with MFP tumors; mice with LAW tumors fed diets containing 1, 2, or 4% 18:2 had 52-69% fewer nodules than similar mice fed diets containing 8 or 12% 18:2. There were no significant differences in the rate of increase of body weight or the daily mean tumor volumes when compared with dietary 18:2 level. Fatty acid composition of the tumor, particularly the level of 18:2, was significantly altered by diet. This study demonstrates that while the level of dietary 18:2 does not enhance the growth rate of primary 4526 tumors and does or does not affect the latency depending on the primary site, it does significantly alter the metastasis. These results stress the importance of metastasis assessment in future studies involving dietary fat effects on tumorigenesis.
研究了膳食中亚油酸（18:2）的定量差异对4526小鼠乳腺肿瘤转移及发展的影响。通过使用椰子油和红花油的混合物制备高脂肪饮食（20%，w/w），其重量含1%、2%、4%、8%或12%的18:2，并喂食雌性BALB/c小鼠，随后在腹壁外侧（LAW）或乳腺脂肪垫（MFP）皮下接种10⁴个4526肿瘤细胞。LAW肿瘤的潜伏期受膳食18:2水平的影响，而MFP肿瘤的潜伏期不受影响。评估转移情况时，喂食含1%、2%、4%或8% 18:2饮食的MFP肿瘤小鼠，其肺表面肿瘤结节比喂食含12% 18:2饮食的类似小鼠少62 - 73%。所有膳食组中患有LAW肿瘤的小鼠比患有MFP肿瘤的小鼠转移到肺部的结节更少；喂食含1%、2%或4% 18:2饮食的LAW肿瘤小鼠比喂食含8%或12% 18:2饮食的类似小鼠结节少52 - 69%。与膳食18:2水平相比，体重增加率或每日平均肿瘤体积没有显著差异。肿瘤的脂肪酸组成，特别是18:2的水平，因饮食而显著改变。本研究表明，虽然膳食18:2水平不会提高原发性4526肿瘤的生长速率，并且根据原发部位对潜伏期有或没有影响，但它确实会显著改变转移情况。这些结果强调了在未来涉及膳食脂肪对肿瘤发生影响的研究中评估转移情况的重要性。
