DSM-BIOMIN Research Center, Technopark 1, 3430 Tulln, Austria.
Molecules. 2023 Jan 19;28(3):1026. doi: 10.3390/molecules28031026.
Industrial farming of livestock is increasingly focused on high productivity and performance. As a result, concerns are growing regarding the safety of food and feed, and the sustainability involved in their production. Therefore, research in areas such as animal health, welfare, and the effects of feed additives on animals is of significant importance. In this study, an in vitro co-culture model of the piglet gut was used to investigate the effects of two phytogenic feed additives (PFA) with similar compositions. Intestinal porcine epithelial cells (IPEC-J2) were co-cultivated with peripheral blood mononuclear cells (PBMC) to model the complex porcine gut environment in vitro. The effects of treatments on epithelial barrier integrity were assessed by means of transepithelial electrical resistance (TEER) in the presence of an inflammatory challenge. Protective effects of PFA administration were observed, depending on treatment duration and the model compartment. After 48 h, TEER values were significantly increased by 12-13% when extracts of the PFA were applied to the basolateral compartment ( < 0.05; n = 4), while no significant effects on cell viability were observed. No significant differences in the activity of a PFA based mainly on pure chemical compounds versus a PFA based mainly on complex, natural essential oils, and extracts were found. Overall, the co-culture model was used successfully to investigate and demonstrate beneficial effects of PFAs on intestinal epithelial barrier function during an inflammatory challenge in vitro. In addition, it demonstrates that the two PFAs are equivalent in effect. This study provides useful insights for further research on porcine gut health status even without invasive in vivo trials.
畜牧业的工业化养殖越来越注重高生产力和高性能。因此，人们对食品和饲料的安全性以及其生产的可持续性的担忧日益增加。因此，动物健康、福利以及饲料添加剂对动物的影响等领域的研究具有重要意义。在这项研究中，使用了仔猪肠道的体外共培养模型来研究两种具有相似成分的植物源饲料添加剂（PFA）的影响。猪肠上皮细胞（IPEC-J2）与外周血单核细胞（PBMC）共培养，以体外模拟复杂的猪肠道环境。通过在存在炎症挑战的情况下测量跨上皮电阻（TEER）来评估处理对上皮屏障完整性的影响。根据处理持续时间和模型隔室的不同，观察到 PFA 给药的保护作用。48 小时后，当将 PFA 的提取物施加到基底外侧隔室时，TEER 值显著增加了 12-13%（<0.05；n=4），而细胞活力没有观察到显著影响。基于纯化学化合物的 PFA 与主要基于复杂天然精油和提取物的 PFA 的活性之间没有发现显著差异。总体而言，共培养模型成功地用于研究和证明了 PFA 在体外炎症挑战期间对肠道上皮屏障功能的有益影响。此外，它表明两种 PFA 的效果相当。这项研究为进一步研究猪肠道健康状况提供了有用的见解，即使没有侵入性的体内试验。
