Bachinger Diana, Mayer Elisabeth, Kaschubek Theresa, Schieder Carina, König Jürgen, Teichmann Klaus
BIOMIN Research Center, Tulln an der Donau, Austria.
Department of Nutritional Science, University of Vienna, Vienna, Austria.
J Anim Physiol Anim Nutr (Berl). 2019 Jan;103(1):210-220. doi: 10.1111/jpn.12997. Epub 2018 Oct 23.
The gut barrier is essential for animal health as it prevents the passage of potentially harmful foreign substances. The epithelial tight junctions support the intestinal barrier and can be disrupted by stress caused, for example, by pathogens or dietary or environmental factors, predisposing the host to disease. In animal husbandry, phytogenics (plant-derived feed additives) are used to support and maintain growth, feed efficiency and health. Therefore, several phytogenics were tested in vitro for their influence on the barrier function recovery of intestinal porcine epithelial cells (IPEC-J2) after disruption, particularly on the abundance of tight junction proteins.
IPEC-J2 treated with 1,000 µg/ml liquorice root extract, 80 µg/ml plant powder mix, or 80 µg/ml angelica root powder showed significantly higher trans-epithelial electric resistance (TEER) 24 hr after tight junction disruption via a calcium switch assay than the control. In contrast, cells treated with 1,000 µg/ml oak bark extract showed a significantly lower TEER after 6 hr but not at later time points. The increased TEER caused by the liquorice root extract correlated with an increase in the abundance of the tight junction protein claudin-4.
This study suggests potential beneficial effects of liquorice and angelica root extracts on the gut barrier function when used as feed additives for livestock. Further studies, especially in vivo, are necessary to confirm these findings.
肠道屏障对动物健康至关重要，因为它可防止潜在有害的外来物质通过。上皮紧密连接支持肠道屏障，并且可能会因病原体、饮食或环境因素等引起的应激而被破坏，使宿主易患疾病。在畜牧业中，植物源物质（植物源性饲料添加剂）用于支持和维持生长、饲料效率及健康。因此，对几种植物源物质进行了体外测试，以研究它们对猪肠道上皮细胞（IPEC-J2）紧密连接破坏后屏障功能恢复的影响，特别是对紧密连接蛋白丰度的影响。
通过钙切换试验破坏紧密连接后24小时，用1000μg/ml甘草根提取物、80μg/ml植物粉末混合物或80μg/ml当归根粉末处理的IPEC-J2的跨上皮电阻（TEER）显著高于对照组。相比之下，用1000μg/ml橡树皮提取物处理的细胞在6小时后TEER显著降低，但在随后的时间点没有降低。甘草根提取物引起的TEER增加与紧密连接蛋白claudin-4丰度的增加相关。
本研究表明，甘草和当归根提取物作为家畜饲料添加剂使用时，对肠道屏障功能可能具有有益作用。需要进一步研究，尤其是体内研究，以证实这些发现。
