Salem Mohamed Z M, Mohamed Abeer A, Ali Hayssam M, Al Farraj Dunia A
Forestry and Wood Technology Department, Faculty of Agriculture (El-Shatby), Alexandria University, Alexandria 21545, Egypt.
Plant Pathology Institute, Agriculture Research Center (ARC), Alexandria 21616, Egypt.
Plants (Basel). 2021 Jun 29;10(7):1325. doi: 10.3390/plants10071325.
Trees are good sources of bioactive compounds as antifungal and antioxidant activities.
Management of six molecularly identified isolates (F. oxy 1, F. oxy 2, F. oxy 3, F. oxy 4, F. oxy 5 and F. oxy 6, under the accession numbers MW854648, MW854649, MW854650, MW854651, and MW854652, respectively) was assayed using four extracts from leaves, bark, wood and leaves. All the extracts were analyzed using HPLC-VWD for phenolic and flavonoid compounds and the antioxidant activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and -carotene-linoleic acid (BCB) bleaching assays.
In mg/kg extract, the highest amounts of polyphenolic compounds -hydroxy benzoic, benzoic, gallic, and rosmarinic acids, with 444.37, 342.16, 311.32 and 117.87, respectively, were observed in leaf extract; gallic and benzoic acids with 2551.02, 1580.32, respectively, in bark extract; quinol, naringenin, rutin, catechol, and benzoic acid with 2530.22, 1224.904, 798.29, 732.28, and 697.73, respectively, in wood extract; and rutin, -coumaric acid, -hydroxy benzoic acid, resveratrol, and rosmarinic acid with 9168.03, 2016.93, 1009.20, 1156.99, and 574.907, respectively, in leaf extract. At the extract concentration of 1250 mg/L, the antifungal activity against the growth of strains showed that bark followed by leaf extracts had the highest inhibition percentage of fungal growth (IPFG%) against F. oxy 1 with 80% and 79.5%, F. oxy 2 with 86.44% and 78.9%, F. oxy 3 with 86.4% and 84.2%, F. oxy 4 with 84.2, and 82.1%, F. oxy 5 with 88.4% and 86.9%, and F. oxy 6 with 88.9, and 87.1%, respectively. For the antioxidant activity, ethanolic extract from leaves showed the lowest concentration that inhibited 50% of DPPH free radical (3.4 μg/mL). Additionally, the same extract observed the lowest concentration (4.5 μg/mL) that inhibited BCB bleaching.
Extracts from bark and leaves are considered potential candidates against the growth of isolates-a wilt pathogen-and leaf as a potent antioxidant agent.
树木是具有抗真菌和抗氧化活性的生物活性化合物的良好来源。
使用从树叶、树皮、木材和树叶中提取的四种提取物，对六个经分子鉴定的分离株（尖孢镰刀菌1、尖孢镰刀菌2、尖孢镰刀菌3、尖孢镰刀菌4、尖孢镰刀菌5和尖孢镰刀菌6，登录号分别为MW854648、MW854649、MW854650、MW854651和MW854652）进行处理。所有提取物均使用高效液相色谱 - 可变波长检测器（HPLC-VWD）分析酚类和黄酮类化合物，并使用2,2-二苯基-1-苦基肼（DPPH）自由基清除法和β-胡萝卜素-亚油酸（BCB）漂白试验评估抗氧化活性。
以毫克/千克提取物计，在树叶提取物中观察到多酚类化合物（对羟基苯甲酸、苯甲酸、没食子酸和迷迭香酸）含量最高，分别为444.37、342.16、311.32和117.87；在树皮提取物中没食子酸和苯甲酸含量分别为2551.02和1580.32；在木材提取物中喹诺、柚皮素、芦丁、儿茶酚和苯甲酸含量分别为2530.22、1224.904、798.29、732.28和（此处原文有误，推测应为697.73）697.73；在树叶提取物中芦丁、对香豆酸、对羟基苯甲酸、白藜芦醇和迷迭香酸含量分别为9168.03、2016.93、1009.20、1156.99和574.907。在提取物浓度为1250毫克/升时，针对菌株生长的抗真菌活性表明，树皮提取物其次是树叶提取物对尖孢镰刀菌1的真菌生长抑制率（IPFG%）最高，分别为80%和79.5%；对尖孢镰刀菌2分别为86.44%和78.9%；对尖孢镰刀菌3分别为86.4%和84.2%；对尖孢镰刀菌4分别为84.2%和82.1%；对尖孢镰刀菌5分别为88.4%和86.9%；对尖孢镰刀菌6分别为88.9%和87.1%。对于抗氧化活性，树叶乙醇提取物显示出抑制50% DPPH自由基的最低浓度（3.4微克/毫升）。此外，相同提取物观察到抑制BCB漂白的最低浓度（4.5微克/毫升）。
树皮和树叶提取物被认为是对抗分离株（一种枯萎病原体）生长的潜在候选物，树叶是一种有效的抗氧化剂。
