College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Anxi College of Tea Science, Fujian Agriculture and Forestry University, Quanzhou 362400, China.
Molecules. 2023 Jan 23;28(3):1124. doi: 10.3390/molecules28031124.
Lanthanum can affect the growth and development of the tea plant. Tieguanyin (TGY) and Shuixian (SX) cultivars of Camellia sinensis were selected to explore the mechanism underlying the accumulation of lanthanum (tea plants' most accumulated rare earth element) through proteomics. Roots and fresh leaves of TGY and SX with low- and high-accumulation potential for lanthanum, respectively, were studied; 845 differentially expressed proteins (DEPs) were identified. Gene ontology analysis showed that DEPs were involved in redox processes and related to molecular functions. Kyoto Encyclopedia of Genes and Genomes metabolic pathway analysis showed that DEPs were associated with glutathione (GSH) and α-linolenic acid metabolism, plant pathogen interaction, and oxidative phosphorylation. Thirty-seven proteins in the GSH metabolism pathway showed significant differences, wherein 18 GSH S-transferases showed differential expression patterns in the root system. Compared with the control, expression ratios of GST (TEA004130.1) and GST (TEA032216.1) in TGY leaves were 6.84 and 4.06, respectively, after lanthanum treatment; these were significantly higher than those in SX leaves. The LOX2.1 (TEA011765.1) and LOX2.1 (TEA011776.1) expression ratios in the α-linolenic acid metabolic pathway were 2.44 and 6.43, respectively, in TGY roots, which were significantly higher than those in SX roots. The synthesis of specific substances induces lanthanum-associated defense responses in TGY, which is of great significance for plant yield stability.
镧可能会影响茶树的生长和发育。选择铁观音（TGY）和水仙（SX）两个茶树品种，通过蛋白质组学探索茶树中镧（植物积累量最大的稀土元素）积累的机制。研究了镧低积累和高积累潜力的 TGY 和 SX 的根和新鲜叶片，共鉴定到 845 个差异表达蛋白（DEPs）。GO 分析表明，DEPs 参与了氧化还原过程，与分子功能有关。KEGG 代谢途径分析表明，DEPs 与谷胱甘肽（GSH）和α-亚麻酸代谢、植物病原体相互作用和氧化磷酸化有关。GSH 代谢途径中的 37 种蛋白表现出显著差异，其中 18 种 GSH S-转移酶在根系中的表达模式存在差异。与对照相比，镧处理后 TGY 叶片中 GST（TEA004130.1）和 GST（TEA032216.1）的表达比例分别为 6.84 和 4.06，均显著高于 SX 叶片；LOX2.1（TEA011765.1）和 LOX2.1（TEA011776.1）在α-亚麻酸代谢途径中的表达比例分别为 2.44 和 6.43，均显著高于 SX 根。特定物质的合成诱导了 TGY 中与镧相关的防御反应，这对于植物产量稳定性具有重要意义。
