叶黄素异构体：制备、分离、结构解析及 20 种药用植物中的分布。

Lutein Isomers: Preparation, Separation, Structure Elucidation, and Occurrence in 20 Medicinal Plants.

机构信息

Department of Biochemistry and Medical Chemistry, Medical School, University of Pécs, Szigeti út 12., H-7624 Pécs, Hungary.

Department of Pharmacognosy, Faculty of Pharmacy, University of Pécs, Rókus u. 2., H-7624 Pécs, Hungary.

出版信息

Molecules. 2023 Jan 25;28(3):1187. doi: 10.3390/molecules28031187.

DOI:10.3390/molecules28031187

PMID:36770852

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9921531/

Abstract

Lutein and its -isomers occur in a lot of plants, including a variety of flowers. In this study, lutein isomers were produced via iodine-catalyzed isomerization, and four -isomers (9-, 9'-, 13-, and 13') were isolated by means of column chromatography and semipreparative HPLC. The structures of the 9'- and 13'-isomers were elucidated via NMR measurements. These compounds were used as standards for the HPLC-DAD-MS determination of the carotenoid composition of the flowers of 20 plant species, in which lutein and its geometrical isomers are the main components. The flowers showed great variation in their - and -lutein content, and also in the presence or absence of other carotenoids, such as violaxanthin, neoxanthin, β-cryptoxanthin, and β-carotene. Some of the investigated flowers were found to be rich sources of lutein without zeaxanthin.

摘要

叶黄素及其异构体存在于许多植物中，包括各种花卉。在这项研究中，通过碘催化异构化生产叶黄素异构体，通过柱色谱和半制备 HPLC 分离出四种异构体（9-、9'-、13-和 13'-异构体）。通过 NMR 测量阐明了 9'-和 13'-异构体的结构。这些化合物被用作 HPLC-DAD-MS 测定 20 种植物花中类胡萝卜素组成的标准品，其中叶黄素及其几何异构体是主要成分。这些花在 - 和 - 叶黄素含量以及其他类胡萝卜素（如 violaxanthin、neoxanthin、β-隐黄质和 β-胡萝卜素）的存在或不存在方面存在很大差异。一些被调查的花卉被发现是富含叶黄素而不含玉米黄质的良好来源。