Department of Molecular Biology, Faculty of Medicine, The John Paul II Catholic University of Lublin, Konstantynów 1h, 20-708 Lublin, Poland.
Institute of Molecular Physics Polish Academy of Science, M. Smoluchowskiego 17, 60-179 Poznań, Poland.
Molecules. 2023 Jan 25;28(3):1194. doi: 10.3390/molecules28031194.
A new type of silver nanoparticles (AgNPs) was prepared and comprehensively studied. Scanning electron microscopy (SEM) and dynamic light scattering (DLS) analyses indicated that 24 nm AgNPs with narrow size distribution were obtained while Z-potential confirms their good stability. The composites of the obtained AgNPs with nontoxic-nature-inspired hydrogel were formed upon cooling of the aqueous solution AgNPs and CAla. The thermal gravimetric analysis (TGA) and the differential scanning calorimetry (DSC) do not show significant shifts in the characteristic temperature peaks for pure and silver-enriched gels, which indicates that AgNPs do not strongly interact with CAla fibers, which was also confirmed by SEM. Both AgNPs alone and in the assembly with the gelator CAla were almost biologically passive against bacteria, fungus, cancer, and nontumor human cells, as well as zebra-fish embryos. These studies proved that the new inactive AgNPs-doped hydrogels have potential for the application in therapy as drug delivery media.
一种新型的银纳米粒子（AgNPs）被制备并进行了全面研究。扫描电子显微镜（SEM）和动态光散射（DLS）分析表明，获得了具有较窄尺寸分布的 24nm AgNPs，而 Zeta 电位证实了其良好的稳定性。所得 AgNPs 与无毒的天然启发水凝胶的复合材料是通过冷却含 AgNPs 和 CAla 的水溶液形成的。热重分析（TGA）和差示扫描量热法（DSC）没有显示出纯凝胶和富含银的凝胶的特征温度峰的显著位移，这表明 AgNPs 与 CAla 纤维没有强烈相互作用，这也通过 SEM 得到了证实。单独的 AgNPs 以及与凝胶剂 CAla 的组装体对细菌、真菌、癌症和非肿瘤人类细胞以及斑马鱼胚胎几乎没有生物活性。这些研究证明，新型的非活性 AgNPs 掺杂水凝胶具有作为药物输送介质在治疗中的应用潜力。
