School of Chemical Engineering & Physics Laboratory, Faculty of Engineering, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
Laboratory of Organic Chemistry, School of Chemical Engineering, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
Molecules. 2023 Jan 26;28(3):1221. doi: 10.3390/molecules28031221.
A novel, inexpensive and simple experimental setup for collecting -Raman spectra of volatile liquids in very small quantities was developed. It takes advantage of capillary forces to detain minute volatile liquid volumes. Spectra of volatile and even scattering or absorbing media can be measured more effectively. The method is used to facilitate the collection of intensity-consistent Raman spectra from a series of reference compounds present in essential oils, in order to quantify their constituents by multiple linear regression. Wild grown plants, collected from five different regions in Greece and taxonomically identified as , subsp and subsp. , were appropriately distilled to acquire their essential oils. Comparison of the Raman results with those from headspace gas chromatography-mass spectrometry (HS GC-MS) confirmed the successful relative quantification of the most abundant essential oil constituents, highlighting the similarities and differences of the three taxa examined. Finally, it is demonstrated that directly measuring the leaf peltate glandular hairs yields exploitable results to identify the main components of the essential oil they contain, underlining the potential of in situ (field or industry) measurements utilizing microscope-equipped portable Raman spectrometers.
开发了一种新颖、廉价且简单的实验装置，用于收集非常少量挥发性液体的拉曼光谱。它利用毛细作用力来保留微量挥发性液体。可以更有效地测量挥发性甚至散射或吸收介质的光谱。该方法用于从存在于精油中的一系列参考化合物中收集强度一致的拉曼光谱，以便通过多元线性回归定量它们的成分。从希腊五个不同地区采集的野生生长的植物，经分类鉴定为、和，适当蒸馏以获得它们的精油。拉曼结果与顶空气相色谱-质谱法（HS GC-MS）的结果进行比较，证实了最丰富的精油成分的成功相对定量，突出了所检查的三个 Taxa 的相似性和差异性。最后，证明直接测量叶盾状腺毛可以产生可利用的结果，以鉴定它们所含精油的主要成分，强调了利用配备显微镜的便携式拉曼光谱仪进行原位（现场或工业）测量的潜力。
