Department of Chemistry, University of Agriculture, Faisalabad, Pakistan.
Department of Chemistry, University of Agriculture, Faisalabad, Pakistan.
Spectrochim Acta A Mol Biomol Spectrosc. 2017 Jul 5;182:168-174. doi: 10.1016/j.saa.2017.03.072. Epub 2017 Apr 1.
In this study, Raman spectroscopy along with Principal Component Analysis (PCA) is used for the characterization of pure essential oil (pure EO) isolated from the leaves of the Hemp (Cannabis sativa L.,) as well as its different fractions obtained by fractional distillation process. Raman spectra of pure Hemp essential oil and its different fractions show characteristic key bands of main volatile terpenes and terpenoids, which significantly differentiate them from each other. These bands provide information about the chemical composition of sample under investigation and hence can be used as Raman spectral markers for the qualitative monitoring of the pure EO and different fractions containing different active compounds. PCA differentiates the Raman spectral data into different clusters and loadings of the PCA further confirm the biological origin of the different fractions of the essential oil.
在这项研究中，拉曼光谱结合主成分分析（PCA）用于表征从大麻（Cannabis sativa L.）叶中分离得到的纯精油（纯 EO）及其通过分馏过程获得的不同馏分。纯大麻精油及其不同馏分的拉曼光谱显示出主要挥发性萜类和萜烯的特征关键带，这使得它们彼此之间明显不同。这些带提供了有关所研究样品化学成分的信息，因此可作为拉曼光谱标记物，用于定性监测纯 EO 和含有不同活性化合物的不同馏分。PCA 将拉曼光谱数据分为不同的聚类，PCA 的加载进一步证实了精油不同馏分的生物学来源。
