Flow Innovation-Research for Science and Technology Laboratories (Firstlabs), Ratchathewi District, Bangkok 10110, Thailand.
Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahidol University, Rama 6 Road, Ratchathewi District, Bangkok 10400, Thailand.
Molecules. 2023 Jan 30;28(3):1316. doi: 10.3390/molecules28031316.
This work presents the use of a transparent 'Cross Injection Analysis' (CIA) platform as a flow system for chemiluminescence (CL) measurements. The CL-CIA flow device incorporates introduction channels for samples and reagents, and the reaction and detection channels are in one acrylic unit. A photomultiplier tube placed above the reaction channel detects the emitted luminescence. The system was applied to the analysis of (i) Co(II) via the Co(II)-catalyzed HO-luminol reaction and (ii) paracetamol via its inhibitory effect on the catalytic activity of Fe(CN) on the HO-luminol reaction. A linear calibration was obtained for Co(II) in the range of 0.002 to 0.025 mg L Co(II) (r = 0.9977) for the determination of Co(II) in water samples. The linear calibration obtained for the paracetamol was 10 to 200 mg L (r = 0.9906) for the determination of pharmaceutical products. The sample throughput was 60 samples h. The precision was ≤4.2% RSD. The consumption of the samples and reagents was ca. 170 µL per analysis cycle.
本工作展示了一种透明的“交叉注入分析”（CIA）平台在化学发光（CL）测量中的应用。CL-CIA 流路装置包含样品和试剂的引入通道，反应和检测通道在一个亚克力单元中。光电倍增管放置在反应通道上方，用于检测发射的荧光。该系统用于分析（i）Co(II)，通过 Co(II)催化的 HO-鲁米诺反应，以及（ii）对乙酰氨基酚，通过其对 HO-鲁米诺反应中 Fe(CN)催化活性的抑制作用。在水样品中 Co(II)的测定中，Co(II)的线性校准范围为 0.002 至 0.025 mg L Co(II)（r = 0.9977）。对于药物产品的测定，获得的对乙酰氨基酚的线性校准范围为 10 至 200 mg L（r = 0.9906）。样品的吞吐量为 60 个样品 h。精密度≤4.2%RSD。每个分析循环的样品和试剂消耗量约为 170 µL。
