通过抑制鲁米诺 - H₂O₂ - Fe(CN)₃⁶⁻化学发光体系间接测定药物制剂中的对乙酰氨基酚。

Indirect determination of paracetamol in pharmaceutical formulations by inhibition of the system luminol-H2O2-Fe(CN)3-(6) chemiluminescence.

作者信息

Alapont A G, Zamora L L, Calatayud J M

机构信息

Departamento de Química, Colegio Universitario CEU San Pablo, Moncada, Valencia, Spain.

出版信息

J Pharm Biomed Anal. 1999 Nov;21(2):311-7. doi: 10.1016/s0731-7085(99)00124-7.

DOI:10.1016/s0731-7085(99)00124-7

PMID:10703985

Abstract

After a large drug scanning, the system Luminol-H2O2-Fe(CN)6(3-) is proposed for first time for the indirect determination of paracetamol. The method is based on the oxidation of paracetamol by hexacyanoferrate (III) and the subsequent inhibitory effect on the reaction between luminol and hydrogen peroxide. The procedure resulted in a linear calibration graph over the range 2.5-12.5 microg ml(-1) of paracetamol with a sample throughput of 87 samples h(-1). The influence of foreign compounds was studied and, the method was applied to determination of the drug in three different pharmaceutical formulations.

摘要

经过大规模药物扫描后，首次提出了鲁米诺-H₂O₂-Fe(CN)₆³⁻体系用于间接测定对乙酰氨基酚。该方法基于对乙酰氨基酚被铁氰化铁（III）氧化，以及随后对鲁米诺与过氧化氢之间反应的抑制作用。该程序在2.5-12.5微克/毫升的对乙酰氨基酚范围内产生了线性校准曲线，样品通量为87个样品/小时。研究了外来化合物的影响，并将该方法应用于三种不同药物制剂中药物的测定。