USDA-APHIS-PPQ, Plant Germplasm Quarantine Program, Beltsville, MD, 20705, USA.
Department of Agriculture, Agribusiness, and Environmental Sciences, Texas A&M University-Kingsville, Kingsville, TX, 78363, USA.
Arch Virol. 2023 Feb 11;168(3):86. doi: 10.1007/s00705-023-05708-7.
A new positive-strand RNA virus was discovered in a horse nettle plant, using high-throughput sequencing (HTS), and its complete genome, consisting of RNA1 and RNA2, which are 7522 and 4710 nucleotides in length, respectively, was characterized. Each genome segment contains a single open reading frame flanked by 5' and 3' untranslated regions (UTRs), followed by a poly(A) tail at the 3' end. The encoded proteins have the highest amino acid sequence identity (55% and 45%) to the polyprotein encoded by RNA1 of tomato black ring virus (TBRV) and RNA2 of potato virus B (PVB), respectively. Its genome organization and phylogenetic relationship to other nepoviruses suggested that this virus is a novel member of subgroup B, and recombination analysis revealed its evolutionary history within the subgroup. These results suggest the new virus, provisionally named "horse nettle virus A", represents a new species within the genus Nepovirus.
利用高通量测序（HTS），在荨麻科植物中发现了一种新的正链 RNA 病毒，其全长基因组 RNA1 和 RNA2 分别由 7522 和 4710 个核苷酸组成。每个基因组片段包含一个单一的开放阅读框，侧翼是 5' 和 3' 非翻译区（UTR），3' 末端带有 poly(A) 尾巴。编码蛋白与番茄黑环病毒（TBRV）RNA1 和马铃薯病毒 B（PVB）RNA2 编码的多蛋白具有最高的氨基酸序列同一性（分别为 55%和 45%）。其基因组组织和与其他尼派病毒的系统发育关系表明，该病毒是 B 亚组的一个新成员，重组分析揭示了其在亚组内的进化历史。这些结果表明，这种新病毒，暂时命名为“荨麻科病毒 A”，代表尼派病毒属中的一个新种。
