Wu Zhen-Xing, Wang Jian, Lin Xiao-Hu, Yang Qing, Wang Ting-Zhen, Chen Jian-Jian, Li Xiang-Nan, Guan Yuan, Lv Gui-Hua
Institute of Maize and Featured Upland Crops, Zhejiang Academy of Agricultural Sciences, Dongyang, 322100, China.
College of Agronomy and Biotechnology, Hebei Key Laboratory of Crop Stress Biology, Hebei Normal University of Science &Technology, Qinhuangdao, 066000, China.
Environ Sci Pollut Res Int. 2023 Apr;30(17):49290-49300. doi: 10.1007/s11356-023-25777-0. Epub 2023 Feb 11.
To reduce the harmful effects of nicosulfuron on sweet corn, the physiological regulation mechanism of sweet corn detoxification was studied. This study analyzed the effects of nicosulfuron stress on the glyoxalase system, hormone content, and key gene expression of nicosulfuron-tolerant "HK301" and nicosulfuron-sensitive "HK320" sweet corn seedling sister lines. After spraying nicosulfuron, the methylglyoxal (MG) content in HK301 increased first and then decreased. Glyoxalase I (GlyI) and glyoxalase II (GlyII) activities, non-enzymatic glutathione (GSH), and the glutathione redox state glutathione/(glutathione + glutathione disulfide) (GSH/(GSH + GSSG)) showed a similar trend as the MG content. Abscisic acid (ABA), gibberellin (GA), and zeatin nucleoside (ZR) also increased first and then decreased, whereas the auxin (IAA) increased continuously. In HK301, all indices after spraying nicosulfuron were significantly greater than those of the control. In HK320, MG accumulation continued to increase after nicosulfuron spraying and GlyI and GlyII activities, and GSH first increased and then decreased after 1 day of stress. The indicators above were significantly greater than the control. The GSH/(GSH + GSSG) ratio showed a decreasing trend and was significantly smaller than the control. Furthermore, ABA and IAA continued to increase, and the GA and ZR first increased and then decreased. Compared with HK320, HK301 significantly upregulated the transcription levels of GlyI and GlyII genes in roots, stems, and leaves. Comprehensive analysis showed that sweet maize seedlings improved their herbicide resistance by changing the glyoxalase system and regulating endogenous hormones. The results provide a theoretical basis for further understanding the response mechanism of the glyoxalase system and the regulation characteristics of endogenous hormones in maize under nicosulfuron stress.
为降低烟嘧磺隆对甜玉米的有害影响，研究了甜玉米解毒的生理调控机制。本研究分析了烟嘧磺隆胁迫对耐烟嘧磺隆的“HK301”和对烟嘧磺隆敏感的“HK320”甜玉米幼苗姊妹系的乙二醛酶系统、激素含量和关键基因表达的影响。喷施烟嘧磺隆后，HK301中甲基乙二醛（MG）含量先升高后降低。乙二醛酶I（GlyI）和乙二醛酶II（GlyII）活性、非酶促谷胱甘肽（GSH）以及谷胱甘肽氧化还原状态谷胱甘肽/（谷胱甘肽+谷胱甘肽二硫化物）（GSH/（GSH+GSSG））与MG含量呈现相似趋势。脱落酸（ABA）、赤霉素（GA）和玉米素核苷（ZR）也先升高后降低，而生长素（IAA）持续升高。在HK301中，喷施烟嘧磺隆后的所有指标均显著高于对照。在HK320中，喷施烟嘧磺隆后MG积累持续增加，GlyI和GlyII活性以及GSH在胁迫1天后先升高后降低。上述指标显著高于对照。GSH/（GSH+GSSG）比值呈下降趋势，且显著低于对照。此外，ABA和IAA持续升高，GA和ZR先升高后降低。与HK320相比，HK301显著上调了根、茎和叶中GlyI和GlyII基因的转录水平。综合分析表明，甜玉米幼苗通过改变乙二醛酶系统和调节内源激素来提高其抗除草剂能力。研究结果为进一步了解玉米在烟嘧磺隆胁迫下乙二醛酶系统的响应机制和内源激素的调控特性提供了理论依据。
