College of Landscape and Tourism, Hebei Agricultural University, Baoding, China.
College of Agronomy and Biotechnology, Hebei Key Laboratory of Crop Stress Biology, Hebei Normal University of Science &Technology, Qinhuangdao, China.
PLoS One. 2022 Oct 21;17(10):e0276606. doi: 10.1371/journal.pone.0276606. eCollection 2022.
The sulfonylurea herbicide nicosulfuron is efficient, harmless and selective at low doses and has been widely used in maize cultivation. In this study, a pair of corn sister lines, HK301 (nicosulfuron-tolerence, NT) and HK320 (nicosulfuron-sensitive, NS), was chosen to study the effect of nicosulfuron on plant growth and sugar metabolism in sweet maize (Zea mays L.) seedlings. All the experimental samples were subjected to treatment with water or 80 mg kg-1 of nicosulfuron when the sweet maize seedlings grew to the four-leaf stage. Nicosulfuron significantly inhibited the growth of NS line. The content of sucrose and the activities of sucrose phosphate synthase and sucrose synthase in the two inbred lines increased differentially under nicosulfuron stress compared with the respective control treatment. After nicosulfuron treatment, the activities of hexokinase and 6-phosphofructokinase and the contents of pyruvic acid and citric acid in NS line decreased significantly compared with those of NT line, while the content of sucrose and activities of sucrose phosphate synthase and sucrose synthase increased significantly. The disruption of sugar metabolism in NS line led to a lower supply of energy for growth. This study showed that the glycolysis pathway and the tricarboxylic acid cycle were enhanced in nicosulfuron-tolerant line under nicosulfuron stress in enhancing the adaptability of sweet maize.
磺酰脲类除草剂烟嘧磺隆在低剂量下高效、无害且具有选择性，已被广泛应用于玉米种植。本研究选用一对玉米姊妹系 HK301（烟嘧磺隆耐性，NT）和 HK320（烟嘧磺隆敏感，NS），研究烟嘧磺隆对甜玉米（Zea mays L.）幼苗生长和糖代谢的影响。当甜玉米幼苗长到四叶期时，所有实验样品均用清水或 80mgkg-1 的烟嘧磺隆处理。与各自的对照处理相比，烟嘧磺隆显著抑制 NS 系的生长。在烟嘧磺隆胁迫下，两个自交系的蔗糖含量以及蔗糖磷酸合酶和蔗糖合酶的活性均有不同程度的增加。与 NT 系相比，NS 系经烟嘧磺隆处理后，己糖激酶和 6-磷酸果糖激酶的活性以及丙酮酸和柠檬酸的含量显著降低，而蔗糖的含量和蔗糖磷酸合酶及蔗糖合酶的活性显著增加。NS 系中糖代谢的破坏导致生长的能量供应减少。本研究表明，在烟嘧磺隆胁迫下，耐烟嘧磺隆系通过增强糖酵解途径和三羧酸循环来增强适应性。
