Zimmermann Julius, Sahm Franziska, Arbeiter Nils, Bathel Henning, Song Zezhong, Bader Rainer, Jonitz-Heincke Anika, van Rienen Ursula
Institute of General Electrical Engineering, University of Rostock, D-18051 Rostock, Germany.
Department of Orthopaedics, Biomechanics and Implant Technology Research Laboratory, Rostock University Medical Center, D-18057 Rostock, Germany.
Bioelectrochemistry. 2023 Jun;151:108395. doi: 10.1016/j.bioelechem.2023.108395. Epub 2023 Feb 3.
Electrical stimulation has received increasing attention for decades for its application in regenerative medicine. Applications range from bone growth stimulation over cartilage regeneration to deep brain stimulation. Despite all research efforts, translation into clinical use has not yet been achieved in all fields. Recent critical assessments have identified limited documentation and monitoring of preclinical in vitro and in vivo experiments as possible reasons hampering clinical translation. In this work, we present experimental and numerical methods to determine the crucial quantities of electrical stimulation such as the electric field or current density. Knowing the stimulation quantities contributes to comprehending the biological response to electrical stimulation and to finally developing a reliable dose-response curve. To demonstrate the methods, we consider a direct contact electrical stimulation experiment that stands representative for a broad class of stimulation experiments. Electrochemical effects are addressed and methods to integrate them into numerical simulations are evaluated. A focus is laid on affordable lab equipment and reproducible open-source software solutions. Finally, clear guidelines to ensure replicability of electrical stimulation experiments are formulated.
几十年来,电刺激因其在再生医学中的应用而受到越来越多的关注。其应用范围从骨生长刺激到软骨再生,再到深部脑刺激。尽管进行了所有的研究工作,但并非所有领域都已实现向临床应用的转化。最近的批判性评估指出,临床前体外和体内实验的文献记录和监测有限,这可能是阻碍临床转化的原因。在这项工作中,我们提出了实验和数值方法来确定电刺激的关键量,如电场或电流密度。了解刺激量有助于理解对电刺激的生物学反应,并最终建立可靠的剂量反应曲线。为了演示这些方法,我们考虑了一个直接接触电刺激实验,该实验代表了一大类刺激实验。研究了电化学效应,并评估了将其纳入数值模拟的方法。重点放在经济实惠的实验室设备和可重现的开源软件解决方案上。最后,制定了确保电刺激实验可重复性的明确指南。
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