Department of Restorative and Prosthetic Dentistry, College of Dentistry, Dar Al Uloom University, Riyadh, Saudi Arabia.
Department of Restorative and Prosthetic Dentistry, College of Dentistry, Dar Al Uloom University, Riyadh, Saudi Arabia.
Photodiagnosis Photodyn Ther. 2023 Jun;42:103326. doi: 10.1016/j.pdpdt.2023.103326. Epub 2023 Feb 10.
The present report assessed the efficacy of curcumin-mediated photodynamic therapy (CUR-mediated PDT) as an adjunct to antifungal gel treatment by evaluating the salivary interleukin-6 (IL-6) and matrix metalloproteinases-8 (MMP-8) levels together with Candida species counts in denture stomatitis (DS) patients.
In total, 50 DS subjects were randomly categorized into 2 groups: Group-1: subjects who received the antifungal gel treatment and Group-2: participants who received CUR-mediated PDT. The Sabourad Dextrose Agar and CHROMAgar were utilized for evaluating Candida species counts, while the Enzyme-Linked Immunosorbent Assay was employed to estimate the salivary levels of IL-6 and MMP-8. All clinical evaluations were performed at the baseline, 1 month, and 2 months.
In total, group-2 subjects showed a significant decrease in Candida albicans (C. albicans) counts on both follow-ups (i.e., 1-month and 2-month) than group-1 participants. C. krusei count also reduced in group-2 subejcts than group-1 participants at the 2nd follow-up as compared to the baseline, nevertheless, a slight increase in C. krusei count was noticed in group-2 subjects at the 2nd follow-up than the 1st follow-up. The salivary IL-6 and MMP-8 levels in both groups reduced significantly at both follow-ups than the baseline. According to the stepwise logistic regression analysis, no statistically significant correlation was observed between Candida species count and other parameters such as age and gender of the patient, duration of DS, and frequency of treatment(s).
CUR-mediated PDT is an efficaciousness therapeutic modality for alleviating Candida species counts on the surface of denture and the palatal mucosa, as well as improving the salivary IL-6 and MMP-8 levels in DS patients.
本报告通过评估唾液白细胞介素 6（IL-6）和基质金属蛋白酶-8（MMP-8）水平以及白色念珠菌（Candida albicans）计数，评估姜黄素介导的光动力疗法（CUR-mediated PDT）作为抗真菌凝胶治疗的辅助手段对义齿性口炎（DS）患者的疗效。
共 50 例 DS 患者随机分为两组：组 1：接受抗真菌凝胶治疗的患者，组 2：接受 CUR-mediated PDT 的患者。萨布罗德葡萄糖琼脂和 CHROMAgar 用于评估念珠菌属物种计数，酶联免疫吸附试验用于估计唾液中 IL-6 和 MMP-8 的水平。所有临床评估均在基线、1 个月和 2 个月进行。
组 2 患者在两次随访（即 1 个月和 2 个月）中白色念珠菌（C. albicans）计数均明显低于组 1 患者。与基线相比，组 2 患者的克柔念珠菌（C. krusei）计数在第二次随访中也减少，但与第一次随访相比，组 2 患者的克柔念珠菌（C. krusei）计数在第二次随访中略有增加。两组患者的唾液 IL-6 和 MMP-8 水平在两次随访中均明显低于基线。根据逐步逻辑回归分析，未观察到念珠菌属物种计数与患者年龄、性别、DS 持续时间和治疗频率等其他参数之间存在统计学显著相关性。
CUR-mediated PDT 是一种有效的治疗方法，可减轻义齿表面和腭黏膜上的念珠菌属物种计数，并改善 DS 患者的唾液 IL-6 和 MMP-8 水平。
