Ahmady Azin Rashidy, Solouk Atefeh, Saber-Samandari Saeed, Akbari Somaye, Ghanbari Hadi, Brycki Bogumil E
Department of Biomedical Engineering, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran; Composites Research Laboratory (CRLab), Amirkabir University of Technology, Tehran, Iran.
Department of Biomedical Engineering, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran.
J Colloid Interface Sci. 2023 May 15;638:616-628. doi: 10.1016/j.jcis.2023.01.139. Epub 2023 Jan 31.
Nanocarrier-based drug delivery systems have been designed into various structures that can effectively prevent cancer progression and improve the therapeutic cancer index. However, most of these delivery systems are designed to be simple nanostructures with several limitations, including low stability and burst drug release features. A nano-in-nano delivery technique is explored to address the aforementioned concerns. Accordingly, this study investigated the release behavior of a novel nanoparticles-in-nanofibers delivery system composed of capsaicin-loaded alginate nanoparticles embedded in polycaprolactone-chitosan nanofiber mats. First, alginate nanoparticles were prepared with different concentrations of cationic gemini surfactant and using nanoemulsion templates. The optimized formulation of alginate nanoparticles was utilized for loading capsaicin and exhibited a diameter of 19.42 ± 1.8 nm and encapsulation efficiency of 98.7 % ± 0.6 %. Likewise, blend polycaprolactone-chitosan nanofibers were prepared with different blend ratios of their solutions (i.e., 100:0, 80:20, 60:40) by electrospinning method. After the characterization of electrospun mats, the optimal nanofibers were employed for embedding capsaicin-loaded alginate nanoparticles. Our findings revealed that embedding capsaicin-loaded alginate nanoparticles in polycaprolactone-chitosan nanofibers, prolonged capsaicin release from 120 h to more than 500 h. Furthermore, the results of in vitro analysis demonstrated that the designed nanoplatform could effectively inhibit the proliferation of MCF-7 human breast cells while being nontoxic to human dermal fibroblasts (HDF). Collectively, the prepared nanocomposite drug delivery platform might be promising for the long-term and controlled release of capsaicin for the prevention and treatment of cancer.
基于纳米载体的药物递送系统已被设计成各种结构，这些结构可以有效阻止癌症进展并提高癌症治疗指数。然而，这些递送系统大多被设计为简单的纳米结构，存在一些局限性，包括稳定性低和药物突发释放的特性。为了解决上述问题，人们探索了一种纳米包纳米的递送技术。因此，本研究考察了一种新型的纳米颗粒-纳米纤维递送系统的释放行为，该系统由嵌入聚己内酯-壳聚糖纳米纤维垫中的载辣椒素海藻酸钠纳米颗粒组成。首先，使用不同浓度的阳离子双子表面活性剂并利用纳米乳液模板制备海藻酸钠纳米颗粒。将优化后的海藻酸钠纳米颗粒制剂用于负载辣椒素，其直径为19.42±1.8纳米，包封率为98.7%±0.6%。同样，通过静电纺丝法制备了具有不同溶液混合比例（即100:0、80:20、60:40）的聚己内酯-壳聚糖共混纳米纤维。在对静电纺丝垫进行表征后，采用最佳纳米纤维来包埋载辣椒素的海藻酸钠纳米颗粒。我们的研究结果表明，将载辣椒素的海藻酸钠纳米颗粒嵌入聚己内酯-壳聚糖纳米纤维中，可使辣椒素的释放时间从120小时延长至500多小时。此外，体外分析结果表明，所设计的纳米平台可有效抑制MCF-7人乳腺癌细胞的增殖，同时对人皮肤成纤维细胞（HDF）无毒。总体而言，所制备的纳米复合药物递送平台在辣椒素的长期控释用于癌症预防和治疗方面可能具有广阔前景。
