山地栽培人参中人参皂苷的增强分析与定量及与园参的比较。
Enhanced profiling and quantification of ginsenosides from mountain-cultivated ginseng and comparison with garden-cultivated ginseng.
作者信息
Qu Hua, Wang Jing, Yao Changliang, Wei Xuemei, Wu Yisong, Cheng Mengzhen, He Xin, Li Jiayuan, Wei Wenlong, Zhang Jianqing, Bi Qirui, Guo De-An
机构信息
School of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing 210023, China; National Engineering Research Center of TCM Standardization Technology, Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
National Engineering Research Center of TCM Standardization Technology, Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, China.
出版信息
J Chromatogr A. 2023 Mar 15;1692:463826. doi: 10.1016/j.chroma.2023.463826. Epub 2023 Jan 24.
Panax ginseng can be generally divided into mountain-cultivated ginseng (MCG) and garden-cultivated ginseng (GCG). The market price of MCG is significantly higher than that of GCG. However, the chemical compositions of MCG and the differences from GCG remained unclear. In this study, an integrated strategy combing an offline two-dimensional liquid chromatography separation, LTQ-orbitrap dual mode acquisition, and Q-trap full quantification/quasi-quantification was proposed to explore and compare the chemical compositions of MCG. Consequently, 559 ginsenosides were characterized, among which 437 ginsenosides were in-depth characterized with α-chain and β-chain annotated. Subsequently, enhanced quantification of 213 ginsenosides was conducted in 57 batches of MCG and GCG. Ginsenosides were found more abundant in MCG than GCG. In addition, 25-year-old MCG could be distinctly differentiated from 15/20-year-old MCG. This strategy facilitated the enhanced profiling and comparison of ginsenosides, improved the quality control tactics of MCG and provided a reference approach for other ginseng related products.
人参一般可分为山参和园参。山参的市场价格明显高于园参。然而，山参的化学成分及其与园参的差异仍不明确。在本研究中，提出了一种结合离线二维液相色谱分离、LTQ-轨道阱双模式采集以及Q-阱全定量/准定量的综合策略，以探索和比较山参的化学成分。结果，鉴定出559种人参皂苷，其中437种人参皂苷得到深入鉴定，并标注了α链和β链。随后，对57批山参和园参中的213种人参皂苷进行了增强定量分析。发现山参中的人参皂苷比园参中的更丰富。此外，25年生的山参可以与15/20年生的山参明显区分开来。该策略有助于增强人参皂苷的分析和比较，改进山参的质量控制策略，并为其他与人参相关的产品提供了一种参考方法。
Zotero 插件