Division of Research and Development, Plant Biotechnology Lab, Lovely Professional University, Phagwara, Punjab, India.
Department of Biotechnology, Lovely Faculty of Technology and Sciences, Lovely Professional University, Phagwara, Punjab, India.
Crit Rev Biotechnol. 2024 Mar;44(2):202-217. doi: 10.1080/07388551.2023.2165900. Epub 2023 Feb 12.
Single-cell approaches are a promising way to obtain high-resolution transcriptomics data and have the potential to revolutionize the study of plant growth and development. Recent years have seen the advent of unprecedented technological advances in the field of plant biology to study the transcriptional information of individual cells by single-cell RNA sequencing (scRNA-seq). This review focuses on the modern advancements of single-cell transcriptomics in plants over the past few years. In addition, it also offers a new insight of how these emerging methods will expedite advance research in plant biotechnology in the near future. Lastly, the various technological hurdles and inherent limitations of single-cell technology that need to be conquered to develop such outstanding possible knowledge gain is critically analyzed and discussed.
单细胞方法是获取高分辨率转录组学数据的一种很有前途的方法，有可能彻底改变植物生长和发育的研究。近年来，植物生物学领域出现了前所未有的技术进步，通过单细胞 RNA 测序（scRNA-seq）来研究单个细胞的转录信息。本综述重点介绍了过去几年中植物单细胞转录组学的现代进展。此外，它还提供了一个新的视角，即这些新兴方法将如何在不久的将来加速植物生物技术的研究进展。最后，还批判性地分析和讨论了开发这种卓越的潜在知识收益所需克服的单细胞技术的各种技术障碍和固有局限性。
