Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium; email:
VIB Center for Plant Systems Biology, 9052 Ghent, Belgium.
Annu Rev Plant Biol. 2021 Jun 17;72:847-866. doi: 10.1146/annurev-arplant-081720-010120. Epub 2021 Mar 17.
Single-cell approaches are quickly changing our view on biological systems by increasing the spatiotemporal resolution of our analyses to the level of the individual cell. The field of plant biology has fully embraced single-cell transcriptomics and is rapidly expanding the portfolio of available technologies and applications. In this review, we give an overview of the main advances in plant single-cell transcriptomics over the past few years and provide the reader with an accessible guideline covering all steps, from sample preparation to data analysis. We end by offering a glimpse of how these technologies will shape and accelerate plant-specific research in the near future.
单细胞方法通过将我们的分析的时空分辨率提高到单个细胞的水平，正在迅速改变我们对生物系统的看法。植物生物学领域已经完全接受了单细胞转录组学，并正在迅速扩展可用技术和应用的组合。在这篇综述中，我们概述了过去几年中植物单细胞转录组学的主要进展，并为读者提供了一个涵盖从样品制备到数据分析的所有步骤的易懂指南。最后，我们展望了这些技术将如何在不久的将来塑造和加速植物特异性研究。
