Otero J R, Amor E, Martin-Rabadan P, Noriega A R
Servicio de Microbiología, Hospital Primero de Octubre, Madrid, Spain.
Diagn Microbiol Infect Dis. 1987 Aug;7(4):279-81. doi: 10.1016/0732-8893(87)90145-3.
Forty-six strains of Staphylococcus aureus and 47 strains of unspeciated coagulase-negative staphylococci were inoculated in tubes containing a cation-supplemented Mueller-Hinton broth with 4% (wt/vol) NaCl. A critical concentration of methicillin (10 micrograms/ml) or oxacillin (6 micrograms/ml) was achieved in each tube by adding the appropriate number of antibiotic disks. Growth was interpreted as resistance. Results obtained with fully resistant or fully sensitive strains showed complete agreement with the reference method. Four strains with intermediate sensitivity (two S. aureus and two coagulase-negative staphylococci) were classified as resistant by the broth-disk elution test.
将46株金黄色葡萄球菌和47株未分类的凝固酶阴性葡萄球菌接种于含有补充阳离子的穆勒-欣顿肉汤且含4%（重量/体积）氯化钠的试管中。通过添加适量数量的抗生素纸片，使每支试管中达到甲氧西林（10微克/毫升）或苯唑西林（6微克/毫升）的临界浓度。生长被判定为耐药。完全耐药或完全敏感菌株所得结果与参考方法完全一致。肉汤-纸片洗脱试验将4株中度敏感菌株（2株金黄色葡萄球菌和2株凝固酶阴性葡萄球菌）判定为耐药。
