Hansen S L, Pope W A
J Clin Microbiol. 1985 Nov;22(5):886-7. doi: 10.1128/jcm.22.5.886-887.1985.
A broth screening method was developed to detect methicillin-resistant (heteroresistant) strains of Staphylococcus aureus from primary plates. Lyophilized vials containing cation-supplemented Mueller-Hinton broth, 2% NaCl, and 10 micrograms of methicillin and 6 micrograms of oxacillin per ml were hydrated and tested with 129 methicillin-resistant and 35 methicillin-susceptible strains of S. aureus. With the addition of a tetrazolium indicator after 5 h of incubation, 96.9% of resistant strains were detected. No false-positive results occurred.
开发了一种肉汤筛选方法，用于从原始平板中检测耐甲氧西林（异质性耐药）金黄色葡萄球菌菌株。将含有补充阳离子的穆勒-欣顿肉汤、2%氯化钠以及每毫升10微克甲氧西林和6微克苯唑西林的冻干小瓶复水，并对129株耐甲氧西林金黄色葡萄球菌菌株和35株甲氧西林敏感金黄色葡萄球菌菌株进行测试。孵育5小时后加入四氮唑指示剂，检测到96.9%的耐药菌株。未出现假阳性结果。
