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γ-氨基丁酸富集前后赤小豆的营养成分转移及抗氧化作用

Nutrient transfer and antioxidant effect of adzuki bean before and after GABA enrichment.

作者信息

Jiang Xiujie, Xu Qingpeng, Zhang Jiayu, Li Zhijiang, Tang Huacheng, Cao Dongmei, Zhang Dongjie

机构信息

College of Food Science, Heilongjiang Bayi Agricultural University, Daqing, China.

National Coarse Cereals Engineering Research Center, Heilongjiang Bayi Agricultural University, Daqing, China.

出版信息

Front Nutr. 2023 Jan 26;10:1123075. doi: 10.3389/fnut.2023.1123075. eCollection 2023.

Abstract

In order to study the nutritional changes of γ-aminobutyric acid (GABA) enrichment in adzuki bean germination, vacuum combined with monosodium glutamate (MSG) was used as the germination stress of adzuki bean. The nutrient transfer before and after GABA enrichment in adzuki bean germination under vacuum combined with MSG stress were studied by means of chromatography and scanning electron microscope (SEM). The antioxidant activity and hypoglycemic effect of different solvent extracts before and after germination of adzuki bean were evaluated by experiments in vitro. The results showed that the nutritional characteristics of adzuki bean rich in GABA changed significantly ( < 0.05), the total fatty acids decreased significantly ( < 0.05), and the 21 amino acids detected increased significantly. After germination, the starch granules of adzuki bean became smaller and the surface was rough Germination stress significantly increased the antioxidant and hypoglycemic activities of the extracts from different solvents ( < 0.05), and the water extracts had the best effect on DPPH and ⋅OH radical scavenging rates of 88.52 and 83.56%, respectively. The results indicated that the germinated adzuki bean rich in GABA was more nutritious than the raw adzuki bean and had good antioxidant activity. It hoped to provide technical reference for rich food containing GABA.

摘要

为研究真空结合味精(MSG)作为赤小豆发芽胁迫对γ-氨基丁酸(GABA)富集过程中营养变化的影响,采用色谱法和扫描电子显微镜(SEM)研究了真空结合MSG胁迫下赤小豆发芽过程中GABA富集前后的营养物质转移情况。通过体外实验评价了赤小豆发芽前后不同溶剂提取物的抗氧化活性和降血糖作用。结果表明,富含GABA的赤小豆营养特性变化显著(P<0.05),总脂肪酸显著降低(P<0.05),检测到的21种氨基酸显著增加。发芽后,赤小豆淀粉颗粒变小,表面粗糙。发芽胁迫显著提高了不同溶剂提取物的抗氧化和降血糖活性(P<0.05),其中水提取物对DPPH和·OH自由基的清除率分别达到88.52%和83.56%,效果最佳。结果表明,富含GABA的发芽赤小豆比未发芽赤小豆更具营养,且具有良好的抗氧化活性。希望为富含GABA的食品提供技术参考。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3daf/9909224/90f145af2eb1/fnut-10-1123075-g001.jpg

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