Department of Plant Science, Gangneung-Wonju National University, Gangneung, Republic of Korea.
Natural Product Research Center, Korea Institute of Science and Technology, Gangneung, Republic of Korea.
PLoS One. 2020 Apr 27;15(4):e0232159. doi: 10.1371/journal.pone.0232159. eCollection 2020.
In this study, we investigated changes in the isoflavone content, total phenolic content (TPC), total flavonoid content (TFC), antioxidant activities (DPPH, ABTS), and anti-inflammatory activities of small-seeded and large-seeded soybean cultivars during germination (light/dark conditions). Total isoflavone content was higher at the seed stage in large-seeded soybeans, while it increased after 7 days of germination in small-seeded soybeans, particularly in response to light conditions, under which they had high TPC, TFC, and antioxidant activities. In large-seeded soybeans, the germination environment did not significantly affect TFC or DPPH inhibition, whereas TPC and ABTS inhibition were high under dark germination conditions. Extracts of sprouts exhibited superior anti-inflammatory activities. Nitric oxide production was slightly lower in small-seeded and large-seeded soybeans germinated under light and dark conditions, respectively. Our findings indicate that germinated soybeans improved nutritionally, and that enhancement of bioactivity under different germination environments could contribute to the selection of appropriate soybean cultivars.
在这项研究中，我们研究了小粒大豆和大粒大豆在发芽过程（光照/黑暗条件）中异黄酮含量、总酚含量（TPC）、总黄酮含量（TFC）、抗氧化活性（DPPH、ABTS）和抗炎活性的变化。大粒大豆的总异黄酮含量在种子阶段较高，而小粒大豆在发芽 7 天后增加，特别是在光照条件下，具有较高的 TPC、TFC 和抗氧化活性。在大粒大豆中，发芽环境对 TFC 或 DPPH 抑制没有显著影响，而在黑暗发芽条件下 TPC 和 ABTS 抑制较高。豆芽提取物表现出优异的抗炎活性。光照和黑暗条件下发芽的小粒和大粒大豆的一氧化氮生成量均略有降低。我们的研究结果表明，发芽的大豆在营养上得到了改善，并且在不同发芽环境下生物活性的增强可能有助于选择合适的大豆品种。
