Antunes Adriana Maria, Nunes Stival Júlio Gabriel, Targueta Cíntia Pelegrineti, de Campos Telles Mariana Pires, Soares Thannya Nascimentos
Laboratório de Genética & Biodiversidade, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brasil.
Programa de Pós Graduação em Genética e Melhoramento de Plantas, Escola de Agronomia, Universidade Federal de Goias, Goiânia, Goiás, Brasil.
Curr Genomics. 2022 Jul 5;23(3):175-181. doi: 10.2174/1389202923666220428101350.
Also known as Simple Sequence Repetitions (SSRs), microsatellites are profoundly informative molecular markers and powerful tools in genetics and ecology studies on plants. This research presents a workflow for developing microsatellite markers using genome skimming. The pipeline was proposed in several stages that must be performed sequentially: obtaining DNA sequences, identifying microsatellite regions, designing primers, and selecting candidate microsatellite regions to develop the markers. Our pipeline efficiency was analyzed using Illumina sequencing data from the non-model tree species Vog. The pipeline revealed 4,382 microsatellite regions and drew 7,411 pairs of primers for . However, a much larger number of microsatellite regions with the potential to develop markers were discovered from our pipeline. We selected 50 microsatellite regions with high potential for developing markers and organized 29 microsatellite regions in sets for multiplex PCR. The proposed pipeline is a powerful tool for fast and efficient development of microsatellite markers on a large scale in several species, especially nonmodel plant species.
微卫星也被称为简单序列重复（SSRs），是植物遗传学和生态学研究中极具信息价值的分子标记和强大工具。本研究提出了一种利用基因组浅层测序开发微卫星标记的工作流程。该流程分几个阶段提出，必须按顺序执行：获取DNA序列、识别微卫星区域、设计引物以及选择候选微卫星区域来开发标记。我们使用来自非模式树种Vog的Illumina测序数据对该流程的效率进行了分析。该流程揭示了4382个微卫星区域，并为其设计了7411对引物。然而，从我们的流程中发现了大量具有开发标记潜力的微卫星区域。我们选择了50个具有高开发标记潜力的微卫星区域，并将29个微卫星区域组合用于多重PCR。所提出的流程是在多个物种，尤其是非模式植物物种中快速高效地大规模开发微卫星标记的强大工具。
