Shaaban Heba
Department of Pharmaceutical Chemistry, College of Clinical Pharmacy, Imam Abdulrahman Bin Faisal University King Faisal Road, P.O. Box 1982 Dammam 31441 Eastern Province Saudi Arabia
RSC Adv. 2023 Feb 9;13(8):5058-5069. doi: 10.1039/d2ra08221g. eCollection 2023 Feb 6.
The greening of pharmaceutical analysis is gaining interest, and different approaches have been proposed, such as minimizing the consumption of hazardous reagents, replacing toxic solvents with safer alternatives, and reducing waste generation. In this work, a natural deep eutectic solvent (NADES) was synthesized and utilized as a green alternative in dispersive liquid-liquid microextraction (DLLME) for the determination of chloramphenicol in honey. Different deep eutectic solvents composed of monoterpenoids and acids were tested. The NADES system composed of menthol and acetic acid at a molar ratio of 1 : 1 was found to be the most appropriate in terms of extraction recovery. Different DLLME parameters including vortex time, centrifugation time, sample volume, and deep eutectic solvent volume were optimized. A determination coefficient of 0.9997 was achieved. Satisfactory recovery ranged from 98.8 to 101.5 with % RSD ≤4.5. The chromatographic performance of the presented method compared with other previously documented methods for determination of chloramphenicol in honey was highlighted. Additionally, the ecological impact of the developed method was assessed employing three tools: the Analytical Eco-scale, the Green Analytical Procedure Index, and the Analytical GREEnness metric. The presented method can be regarded as a green substitute for the traditional methods used for the determination of chloramphenicol in honey.
药物分析的绿色化正引起人们的关注，并且已经提出了不同的方法，例如尽量减少有害试剂的消耗、用更安全的替代品取代有毒溶剂以及减少废物产生。在这项工作中，合成了一种天然深共熔溶剂（NADES），并将其用作分散液液微萃取（DLLME）中的绿色替代品，用于测定蜂蜜中的氯霉素。测试了由单萜类化合物和酸组成的不同深共熔溶剂。发现薄荷醇与乙酸摩尔比为1:1的NADES体系在萃取回收率方面最为合适。优化了包括涡旋时间、离心时间、样品体积和深共熔溶剂体积在内的不同DLLME参数。测定系数达到0.9997。回收率令人满意，范围为98.8%至101.5%（相对标准偏差≤4.5%）。突出了所提出方法与其他先前记录的蜂蜜中氯霉素测定方法相比的色谱性能。此外，使用三种工具评估了所开发方法的生态影响：分析生态尺度、绿色分析程序指数和分析绿色度指标。所提出的方法可被视为测定蜂蜜中氯霉素的传统方法的绿色替代品。
