Jing Yonglian, Bian Liming, Zhang Xuefeng, Zhao Benwen, Zheng Renhua, Su Shunde, Ye Daiquan, Zheng Xueyan, El-Kassaby Yousry A, Shi Jisen
State Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing, China.
Key Laboratory of Timber Forest Breeding and Cultivation for Mountainous Areas in Southern China, Fujian Academy of Forestry Science, Fuzhou, China.
Front Plant Sci. 2023 Jan 27;14:1106615. doi: 10.3389/fpls.2023.1106615. eCollection 2023.
Studying population genetic structure and diversity is crucial for the marker-assisted selection and breeding of coniferous tree species. In this study, using RAD-seq technology, we developed 343,644 high-quality single nucleotide polymorphism (SNP) markers to resolve the genetic diversity and population genetic structure of 233 Chinese fir selected individuals from the 4 cycle breeding program, representing different breeding generations and provenances. The genetic diversity of the 4 cycle breeding population was high with nucleotide diversity ( ) of 0.003, and and of 0.215 and 0.233, respectively, indicating that the breeding population has a broad genetic base. The genetic differentiation level between the different breeding generations and different provenances was low ( < 0.05), with population structure analysis results dividing the 233 individuals into four subgroups. Each subgroup has a mixed branch with interpenetration and weak population structure, which might be related to breeding rather than provenance, with aggregation from the same source only being in the local branches. Our results provide a reference for further research on the marker-assisted selective breeding of Chinese fir and other coniferous trees.
研究群体遗传结构和多样性对于针叶树种的标记辅助选择和育种至关重要。在本研究中，我们利用RAD-seq技术开发了343,644个高质量单核苷酸多态性（SNP）标记，以解析来自4个轮回育种计划的233株杉木选择个体的遗传多样性和群体遗传结构，这些个体代表了不同的育种世代和种源。4个轮回育种群体的遗传多样性较高，核苷酸多样性（）为0.003，分别为0.215和0.233，表明育种群体具有广泛的遗传基础。不同育种世代和不同种源之间的遗传分化水平较低（<0.05），群体结构分析结果将233个个体分为四个亚组。每个亚组都有一个相互渗透且群体结构较弱的混合分支，这可能与育种而非种源有关，仅在本地分支中有来自同一来源的聚集。我们的结果为杉木和其他针叶树的标记辅助选择育种的进一步研究提供了参考。
