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蚯蚓的基因组序列草图

Draft Genome Sequence of the Earthworm .

作者信息

Arumugaperumal Arun, Sudalaimani Dinesh Kumar, Arumugaswami Vaithilingaraja, Sivasubramaniam Sudhakar

机构信息

1Department of Biotechnology, Manonmaniam Sundaranar University, Tirunelveli, Tamilnadu- 627012, India; 2Department of Molecular and Medical Pharmacology, University of California, Los Angeles, California, CA 90095, USA.

出版信息

Curr Genomics. 2022 Jun 10;23(2):118-125. doi: 10.2174/1389202923666220401095626.

DOI:10.2174/1389202923666220401095626
PMID:36778974
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9878837/
Abstract

Earthworms are annelids. They play a major role in agriculture and soil fertility. Vermicompost is the best organic manure for plant crops. is an earthworm well suited for efficient vermicompost production. The worm is also used to study the cell and molecular biology of regeneration, molecular toxicology, developmental biology, ., because of its abilities like high growth rate, rapid reproduction, tolerability toward wide temperature range, and less cost of maintenance. The whole genome has been revealed only for and In the present work, we sequenced the genome of using the Illumina platform and generated 160,684,383 paired-end reads. The reads were assembled into a draft genome of size 488 Mb with 743,870 contigs and successfully annotated 24,599 genes. Further, 208 stem cell-specific genes and 3,432 non-coding genes were identified. The sequence and annotation details were hosted in a web application available at https://sudhakar-sivasubramaniam-labs.shinyapps.io/eudrilus_genome/.

摘要

蚯蚓是环节动物。它们在农业和土壤肥力方面发挥着重要作用。蚯蚓堆肥是植物作物最好的有机肥料。 是一种非常适合高效生产蚯蚓堆肥的蚯蚓。由于其生长速度快、繁殖迅速、对广泛温度范围的耐受性以及维护成本低等能力,这种蚯蚓还被用于研究再生的细胞和分子生物学、分子毒理学、发育生物学等。目前仅揭示了 和 的全基因组。在本研究中,我们使用Illumina平台对 的基因组进行了测序,生成了1.60684383亿对末端配对读数。这些读数被组装成一个大小为488 Mb、有743,870个重叠群的基因组草图,并成功注释了24,599个基因。此外,还鉴定出了208个干细胞特异性基因和3432个非编码基因。序列和注释细节存于一个网络应用程序中,可在https://sudhakar-sivasubramaniam-labs.shinyapps.io/eudrilus_genome/上获取。

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本文引用的文献

1
The transcriptome of anterior regeneration in earthworm Eudrilus eugeniae.在蚯蚓 Eugeniae 的前再生转录组中。
Mol Biol Rep. 2021 Jan;48(1):259-283. doi: 10.1007/s11033-020-06044-8. Epub 2020 Dec 11.
2
Genome and single-cell RNA-sequencing of the earthworm Eisenia andrei identifies cellular mechanisms underlying regeneration.对蚯蚓 Eisenia andrei 的基因组和单细胞 RNA 测序鉴定了再生相关的细胞机制。
Nat Commun. 2020 May 27;11(1):2656. doi: 10.1038/s41467-020-16454-8.
3
Enriched pressmud vermicompost production with green manure plants using Eudrilus eugeniae.
利用大平二号蚯蚓对绿肥植物进行富营养堆肥生产。
Bioresour Technol. 2020 Mar;299:122578. doi: 10.1016/j.biortech.2019.122578. Epub 2019 Dec 10.
4
Prognostic significance of KN motif and ankyrin repeat domains 1 (KANK1) in invasive breast cancer.KN 基序和锚蛋白重复结构域 1(KANK1)在浸润性乳腺癌中的预后意义。
Breast Cancer Res Treat. 2020 Jan;179(2):349-357. doi: 10.1007/s10549-019-05466-8. Epub 2019 Nov 2.
5
Heterogeneous CPU+GPU-Enabled Simulations for DFTB Molecular Dynamics of Large Chemical and Biological Systems.用于大型化学和生物体系的 DFTB 分子动力学的异构 CPU+GPU 加速模拟。
J Chem Theory Comput. 2019 May 14;15(5):2807-2815. doi: 10.1021/acs.jctc.8b01239. Epub 2019 Mar 27.
6
Data on genome annotation and analysis of earthworm .蚯蚓的基因组注释与分析数据。
Data Brief. 2018 Aug 29;20:525-534. doi: 10.1016/j.dib.2018.08.067. eCollection 2018 Oct.
7
DNA structural features of eukaryotic TATA-containing and TATA-less promoters.真核生物含TATA盒和不含TATA盒启动子的DNA结构特征。
FEBS Open Bio. 2017 Feb 16;7(3):324-334. doi: 10.1002/2211-5463.12166. eCollection 2017 Mar.
8
Fifteen new earthworm mitogenomes shed new light on phylogeny within the Pheretima complex.15 条新的蚯蚓线粒体基因组为宽胸蚓复合体的系统发育提供了新的线索。
Sci Rep. 2016 Feb 1;6:20096. doi: 10.1038/srep20096.
9
Timing and Scope of Genomic Expansion within Annelida: Evidence from Homeoboxes in the Genome of the Earthworm Eisenia fetida.环节动物门基因组扩张的时间和范围:来自蚯蚓赤子爱胜蚓基因组中同源异型框的证据。
Genome Biol Evol. 2015 Dec 10;8(1):271-81. doi: 10.1093/gbe/evv243.
10
Bioremediation of heavy metals and petroleum hydrocarbons in diesel contaminated soil with the earthworm: Eudrilus eugeniae.利用蚯蚓(赤子爱胜蚓)对柴油污染土壤中的重金属和石油烃进行生物修复
Springerplus. 2015 Sep 22;4:540. doi: 10.1186/s40064-015-1328-5. eCollection 2015.