State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China.
Agricultural Genomics Institute, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.
Nat Commun. 2020 May 27;11(1):2656. doi: 10.1038/s41467-020-16454-8.
The earthworm is particularly fascinating to biologists because of its strong regenerative capacity. However, many aspects of its regeneration in nature remain elusive. Here we report chromosome-level genome, large-scale transcriptome and single-cell RNA-sequencing data during earthworm (Eisenia andrei) regeneration. We observe expansion of LINE2 transposable elements and gene families functionally related to regeneration (for example, EGFR, epidermal growth factor receptor) particularly for genes exhibiting differential expression during earthworm regeneration. Temporal gene expression trajectories identify transcriptional regulatory factors that are potentially crucial for initiating cell proliferation and differentiation during regeneration. Furthermore, early growth response genes related to regeneration are transcriptionally activated in both the earthworm and planarian. Meanwhile, single-cell RNA-sequencing provides insight into the regenerative process at a cellular level and finds that the largest proportion of cells present during regeneration are stem cells.
蚯蚓因其强大的再生能力而特别引起生物学家的关注。然而，其自然再生的许多方面仍然难以捉摸。在这里，我们报告了在蚯蚓（Eisenia andrei）再生过程中染色体水平的基因组、大规模转录组和单细胞 RNA 测序数据。我们观察到 LINE2 转座元件和与再生功能相关的基因家族（例如 EGFR、表皮生长因子受体）的扩张，特别是在蚯蚓再生过程中表现出差异表达的基因。时间基因表达轨迹确定了转录调节因子，这些因子对于启动再生过程中的细胞增殖和分化可能至关重要。此外，与再生相关的早期生长反应基因在蚯蚓和涡虫中均被转录激活。同时，单细胞 RNA 测序为细胞水平的再生过程提供了深入了解，并发现再生过程中存在的最大比例的细胞是干细胞。
