Department of Knitwear Engineering, BGMEA University of Fashion & Technology (BUFT), Dhaka, Bangladesh.
Department of Fabric Engineering, Bangladesh University of Textiles (BUTEX), Dhaka, Bangladesh.
J Biomater Sci Polym Ed. 2023 Aug;34(11):1517-1538. doi: 10.1080/09205063.2023.2170139. Epub 2023 Feb 16.
The sophisticated new tissue regeneration focused on nanocomposite with different morphologies achieved through advanced manufacturing technology with the inclusion of bio-inscribed materials has piqued the research community's interest. This research aims at developing hybrid bio-nanocomposites with collagen (Col), () oil and chitosan (Cs) by a bi-layered green electrospinning on polyvinyl chloride (PVA) layer in a different ratio for tissue regeneration. Fiber morphologies through scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR), moisture management, tensile test, antibacterial activity, cell cytotoxicity and wound healing through rabbit model of the fabricated hybrid bio-nanocomposites were investigated. It is worth noting that water-soluble Col (above 60% solution) does not form Taylor cones during electrospinning because unable to overcome the surface tension of the solution (viscosity) to form fibers. The results show that water soluble Col (50% solution) to Cs (25% solution) and (25% solution) has good fiber formation with mean diameter 384 ± 27 nm and degree of porosity is 79%. The fast-absorbing and slow-drying hybrid bio-nanocomposites maintain a moist environment for wounds and allowing gaseous exchange for cell migration and proliferation by the synergistic effects of bio-polymers. All of the biopolymers in bio-nanocomposite improve the H-bonds, which accounts for enough tensile strength to withstand cell pulling force. The antibacterial ZOI concentrations against and were 10 and 8 mm, respectively, which appeared to be sufficient to inhibit bacterial action with 100% cell viability (cytotoxicity). The synergistic effects of and Cs improve tissue regeneration, while native Col improves antibacterial activity, and the rabbit model achieves approximately 84% wound closure in only 10 days, which is 1.5 times faster than the control model. So, the fabricated hybrid bio-composites may be useful for skin tissue engineering.
针对不同形态的纳米复合材料的复杂新型组织再生，通过先进的制造技术，结合生物印记材料，引起了研究界的兴趣。本研究旨在通过双层绿色静电纺丝技术，在聚氯乙烯（PVA）层上以不同比例制备胶原（Col）、油和壳聚糖（Cs）的混合生物纳米复合材料，用于组织再生。通过扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）、水分管理、拉伸试验、抗菌活性、细胞细胞毒性和通过兔模型的伤口愈合来研究所制备的混合生物纳米复合材料的纤维形态。值得注意的是，水溶性 Col（高于 60%的溶液）在静电纺丝过程中由于无法克服溶液的表面张力（粘度）而无法形成泰勒锥，因此无法形成纤维。结果表明，水溶性 Col（50%溶液）与 Cs（25%溶液）和 （25%溶液）具有良好的纤维形成能力，平均直径为 384±27nm，孔隙度为 79%。快速吸收和缓慢干燥的混合生物纳米复合材料通过生物聚合物的协同作用保持伤口的潮湿环境，并允许气体交换，促进细胞迁移和增殖。生物纳米复合材料中的所有生物聚合物都改善了氢键，这足以提供足够的拉伸强度来承受细胞的拉力。对 和 的抗菌 ZOI 浓度分别为 10 和 8mm，这似乎足以抑制细菌作用，细胞活力（细胞毒性）为 100%。 和 Cs 的协同作用促进组织再生，而天然 Col 提高了抗菌活性，兔模型仅在 10 天内实现了约 84%的伤口闭合，比对照模型快 1.5 倍。因此，所制备的混合生物复合材料可能对皮肤组织工程有用。
