Escuela de Ingeniería de Materiales, Facultad de Ingeniería, Universidad del Valle, Calle 13 No. 100-00, Santiago de Cali 760032, Colombia.
Grupo de Investigación Biotecnología, Facultad de Ingeniería, Universidad de San Buenaventura Cali, Carrera 122 No. 6-65, Cali 76001, Colombia.
Int J Mol Sci. 2019 Jun 19;20(12):2987. doi: 10.3390/ijms20122987.
Tissue engineering is gaining attention rapidly to replace and repair defective tissues in the human body after illnesses and accidents in different organs. Electrospun nanofiber scaffolds have emerged as a potential alternative for cell regeneration and organ replacement. In this paper, porous membranes, based on nanofibrous chitosan (CS), polyvinyl alcohol (PVA), and graphene oxide (GO), were obtained via electrospinning methodology. Three different formulations were obtained varying GO content, being characterized by Fourier Transform Infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and energy dispersive spectroscopy (EDS). In vitro tests were carried out, consisting of hydrolytic degradation inside simulated biological fluid (SBF), and in vivo tests were carried out, where the material was implanted in Wistar rats' subcutaneous tissue to determine its biocompatibility. The antibacterial activity was tested against Gram-positive bacteria and and against Gram-negative and , by contact of the electrospun nanofiber scaffolds above inoculum bacterial in Müeller Hinton agar with good inhibition only for scaffolds with the higher GO content (1.0%). The results confirmed good biocompatibility of the nanofibrous scaffolds after in vivo tests in Wistar rats, which evidences its high potential in applications of tissue regeneration.
组织工程学在迅速受到关注，以替代和修复人体在不同器官的疾病和事故后受损的组织。电纺纳米纤维支架作为细胞再生和器官替代的一种潜在选择已经出现。在本文中，通过静电纺丝方法获得了基于纳米纤维壳聚糖（CS）、聚乙烯醇（PVA）和氧化石墨烯（GO）的多孔膜。通过傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）和能谱（EDS）对三种不同配方进行了表征，这些配方通过改变 GO 的含量来获得。进行了体外测试，包括在模拟生物液（SBF）内的水解降解，以及体内测试，将材料植入 Wistar 大鼠的皮下组织以确定其生物相容性。通过在含菌接种物的Müeller Hinton 琼脂上接触电纺纳米纤维支架，对革兰氏阳性菌 和革兰氏阴性菌 进行了抗菌活性测试，只有含有较高 GO 含量（1.0%）的支架具有良好的抑制作用。体内试验结果证实了 Wistar 大鼠体内纳米纤维支架具有良好的生物相容性，这证明了其在组织再生应用中的巨大潜力。
