Kharkiv National Medical University, Ukraine.
Georgian Med News. 2022 Dec(333):61-66.
It turned out that the permanent microbiota of surfaces of acrylic bases of complete removable plastic dentures (CRPD) characterized by several features: the presence of aerobic (7) and anaerobic (10) species. Qualitative and quantitative compositions of microbiocenosis in orthopedic treatment stages have been changed. The revealed patterns require further study in the context of the improvement of decolonization of acrylic bases in the stages of orthopedic treatment by CRPD. Deactivation of acrylic bases of CRPD significantly affects the species composition of microbiota, reduces the quantitative parameters of bacterial contamination. The purpose of our work was determination of bacterial contamination of acrylic bases of CRPD in stages of their clinical exploitation and study of changes in the quantitative composition of the acrylic bases microbiota of CRPD under different decontamination modes. Smears from the inner surface of the acrylic base were stained with Gram and microscopic and seeded on blood agar, Endo agar, Chistovich, Saburo. Species belonging to the micro-organisms were identified by the LAHEMA test system and were determined in colonies forming units (CFUs). These data indicate accumulation under the acrylic base in the course of clinical operation of certain types of microbiota, which requires improvement of the methods of their decontamination. We studied the change in the quantitative and specific composition of the microbiota (bacterial contamination) of acrylic bases CRPD under different modes ("A", "B"). Patients in group "A" used the Sideex solution - a two-component system consisting of a liquid component (glutaraldehyde solution) and a powdery activator, mixed before use to obtain a working activated solution. The liquid component is a clear, colorless solution with a specific odor, which is a 2.2-2.7% aqueous solution of glutaraldehyde, which is an active ingredient, pH=3.0 - 4.5. Powdery activator is a pale-yellow powder containing alkaline components, a corrosion inhibitor, and a dye. The activated working solution is a fluorescent green solution with a specific odor and containing 2.2-2.7% glutaraldehyde; pH=8.2-9.2. (Great Britain). Preparation of the activated solution: a powder-activator is added to the container containing the liquid component (avoiding losses). Cleaning is carried out by the method of full immersion of the prosthesis in solution, with a thickness of the drug over it not less than 1 cm. The dentures are soused in solution for 15 minutes, then thorough washing in the same solution for 1-3 minutes. Sideex activated solution is used for sterilization and disinfection of metal, glass, polymeric (plastic, rubber, etc.) medical products. The dentures of patients from group "B" are decontaminated with 0.2% solution of chlorhexidine bigluconate. The dentures were placed in the solution overnight for 14 days, changing the solution every two days. The decontamination of acrylic bases of CRPD should be considered mode "B" as more effective because of the significant decrease in the species composition of the microbiota (the qualitative indicator of reduction of microbial contamination was 72.0%). It is substantiated that decontamination of acrylic bases of CRPD with mode "A" has a small effect on the species composition of the microbiota, although it significantly (p<0.05) reduces quantitative indicators of microbial contamination. Regarding "B" mode, the species composition of aerobes from 31 lgCFU/ml to 8.7 lgCFU/ml and anaerobes from 42.7 lgCFU/ml to 14.6 lgCFU/ml was considered more effective.
结果表明，全口义齿丙烯酸基表面的永久性微生物群具有以下几个特征：存在需氧（7）和厌氧（10）物种。在矫形治疗阶段，微生物群落的定性和定量组成发生了变化。所揭示的模式需要在通过 CRPD 对丙烯酸基进行去定植的改良背景下进一步研究。CRPD 丙烯酸基的失活显著影响微生物群的物种组成，降低细菌污染的定量参数。我们工作的目的是确定 CRPD 丙烯酸基在临床使用阶段的细菌污染，并研究不同去污染模式下 CRPD 丙烯酸基微生物群的定量组成变化。从丙烯酸基的内表面取涂片，用革兰氏染色和显微镜检查，并接种于血琼脂、Endo 琼脂、Chistovich、Saburo 琼脂上。通过 LAHEMA 测试系统鉴定属于微生物的物种，并以菌落形成单位（CFU）确定。这些数据表明，在临床操作过程中，丙烯酸基下会积聚一定类型的微生物群，这需要改进其去污染方法。我们研究了不同模式（“A”、“B”）下 CRPD 丙烯酸基微生物群（细菌污染）的定量和特定组成变化。组“ A”中的患者使用 Sideex 溶液-由液体成分（戊二醛溶液）和粉末状活性剂组成的双组分系统，在使用前混合以获得工作激活溶液。液体成分是一种透明、无色的溶液，具有特定的气味，是一种活性成分，pH 值为 3.0-4.5 的 2.2-2.7%的水溶液。粉末状活性剂是一种浅黄色粉末，含有碱性成分、腐蚀抑制剂和染料。激活的工作溶液是一种带有特定气味且含有 2.2-2.7%戊二醛的荧光绿色溶液；pH 值为 8.2-9.2。（英国）。制备激活溶液：将粉末-活性剂加入装有液体成分的容器中（避免损失）。清洁方法是将义齿完全浸入溶液中，使其厚度超过药物 1 厘米。义齿浸泡在溶液中 15 分钟，然后在相同的溶液中彻底冲洗 1-3 分钟。Sideex 激活溶液可用于金属、玻璃、聚合物（塑料、橡胶等）医疗产品的消毒和消毒。组“ B”的患者的义齿用 0.2%葡萄糖酸氯己定溶液进行消毒。义齿在溶液中放置过夜 14 天，每两天更换一次溶液。CRPD 丙烯酸基的去污应被视为模式“ B”，因为它对微生物群的物种组成有更显著的影响（微生物污染的定性指标减少了 72.0%）。研究表明，模式“A”对丙烯酸基微生物群的物种组成的影响较小，但它可显著（p<0.05）降低微生物污染的定量指标。关于“ B”模式，需氧菌从 31 lgCFU/ml 降低到 8.7 lgCFU/ml，厌氧菌从 42.7 lgCFU/ml 降低到 14.6 lgCFU/ml 被认为更有效。
