Department of Epidemiology and Biostatistics, School of Public Health, Jilin University, No.1163 Xinmin Street, Changchun, Jilin 130021, China.
Cell Immunol. 2023 Mar;385:104689. doi: 10.1016/j.cellimm.2023.104689. Epub 2023 Feb 10.
To investigate the effect conferred by vaccination and previous infection against SARS-CoV-2 infection in molecular level, weighted gene co-expression network analysis was applied to screen vaccination, prior infection and Omicron infection-related gene modules in 46 Omicron outpatients and 8 controls, and CIBERSORT algorithm was used to infer the proportions of 22 subsets of immune cells. 15 modules were identified, where the brown module showed positive correlations with Omicron infection (r = 0.35, P = 0.01) and vaccination (r = 0.62, P = 1 × 10). Enrichment analysis revealed that LILRB2 was the unique gene shared by both phosphatase binding and MHC class I protein binding. Pathways including "B cell receptor signaling pathway" and "FcγR-mediated phagocytosis" were enriched in the vaccinated samples of the highly correlated LILRB2. LILRB2 was also identified as the second hub gene through PPI network, after LCP2. In conclusion, attenuated LILRB2 transcription in PBMC might highlight a novel target in overcoming immune evasion and improving vaccination strategies.
为了从分子水平上探究疫苗接种和既往感染对 SARS-CoV-2 感染的影响，我们应用加权基因共表达网络分析筛选了 46 名奥密克戎门诊患者和 8 名对照者中与疫苗接种、既往感染和奥密克戎感染相关的基因模块，并使用 CIBERSORT 算法推断了 22 种免疫细胞亚群的比例。鉴定出 15 个模块，其中棕色模块与奥密克戎感染呈正相关(r = 0.35，P = 0.01)，与疫苗接种呈正相关(r = 0.62，P = 1 × 10)。富集分析显示，LILRB2 是磷酸酶结合和 MHC Ⅰ类蛋白结合的唯一共享基因。在高相关 LILRB2 的疫苗接种样本中，富集了包括“B 细胞受体信号通路”和“FcγR 介导的吞噬作用”在内的通路。LILRB2 也通过 PPI 网络被鉴定为第二个枢纽基因，仅次于 LCP2。综上所述，PBMC 中 LILRB2 转录的减弱可能突出了克服免疫逃逸和改进疫苗接种策略的一个新靶点。
