Rodrigues Bruna M, Victória Henrique F V, Leite Guilherme, Krambrock Klaus, Chaves Otávio A, de Oliveira Diego F, Garcia Rafael de Q, De Boni Leonardo, Costa Luiz A S, Iglesias Bernardo A
Bioinorganic and Porphyrinoid Material Laboratory, Department of Chemistry, Federal University of Santa Maria, Santa Maria, RS, Brazil.
Department of Physics, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
J Inorg Biochem. 2023 May;242:112149. doi: 10.1016/j.jinorgbio.2023.112149. Epub 2023 Jan 24.
We report the synthesis and characterization of new tri-cationic corrole derivatives, containing Pt(II) or Pd(II) complexes attached at the peripheral position of thienyl moieties. Corrole derivatives were characterized through microanalysis, electrochemical, spectrometry and spectroscopy analysis. Singlet and triplet excited-states are investigated by photophysical/theoretical calculation methods and photobiological parameters were also evaluated spectroscopic techniques (UV-Vis and EPR). Also, the binding capacity of each corrole derivative with nucleic acids (DNA) and human serum albumin (HSA) was determined by UV-Vis, steady-state, and time-resolved fluorescence spectroscopy, combined with molecular docking analysis. Moreover, the new corroles containing peripheral complexes improve their interactions with biomacromolecules, generate reactive oxygen species under light source irradiation studied and has potential for application in photodynamic therapeutic processes.
我们报道了新型三阳离子卟吩衍生物的合成与表征，这些衍生物含有连接在噻吩基部分外围位置的Pt(II)或Pd(II)配合物。通过微量分析、电化学、光谱和波谱分析对卟吩衍生物进行了表征。采用光物理/理论计算方法研究了单重态和三重态激发态，并利用光谱技术（紫外可见光谱和电子顺磁共振）评估了光生物学参数。此外，通过紫外可见光谱、稳态和时间分辨荧光光谱，并结合分子对接分析，测定了每种卟吩衍生物与核酸（DNA）和人血清白蛋白（HSA）的结合能力。此外，含有外围配合物的新型卟吩改善了它们与生物大分子的相互作用，在研究的光源照射下产生活性氧，具有在光动力治疗过程中的应用潜力。
