Department of Chemistry, Federal University of Santa Maria, Av. Roraima, Santa Maria-RS, Brazil.
Department of Chemistry, Federal University of São Carlos, Rod. Washington Luiz, São Carlos-SP, Brazil.
Dalton Trans. 2022 Jan 25;51(4):1646-1657. doi: 10.1039/d1dt03565g.
We report the synthesis and characterization of two novel tetra-cationic porphyrins, containing Pt(II) or Pd(II) polypyridyl complexes attached at the peripheral position of N-macrocycle. Compounds were characterized through elemental analysis, molar conductivity, cyclic voltammetry, and spectroscopy analysis. Photophysical and photobiological parameters were also evaluated. Also, the binding capacity of each porphyrin with human serum albumin (HSA) was determined by UV-Vis, steady-state, and time-resolved fluorescence spectroscopy, combined with molecular docking calculations. The results suggest that the interaction of these compounds is spontaneous, weak to moderate, and probably occurs at site III (subdomain IB) by non-covalent forces, including van der Waals and H-bonding. Moreover, porphyrins containing peripheral complexes improve their interactions with biomolecules, show good photostability, generate reactive oxygen species under white light studied by electron paramagnetic resonance (EPR) analysis, and promote photo-damage of HSA.
我们报告了两种新型四阳离子卟啉的合成与表征，这些卟啉在 N-大环的外围位置连接了 Pt(II) 或 Pd(II) 多吡啶配合物。通过元素分析、摩尔电导率、循环伏安法和光谱分析对化合物进行了表征。还评估了光物理和光生物学参数。此外，通过紫外可见光谱、稳态和时间分辨荧光光谱结合分子对接计算，测定了每种卟啉与人血清白蛋白（HSA）的结合能力。结果表明，这些化合物的相互作用是自发的、弱到中等的，可能通过非共价力（包括范德华力和氢键）发生在 III 位（亚域 IB）。此外，含有外围配合物的卟啉可改善其与生物分子的相互作用，表现出良好的光稳定性，通过电子顺磁共振（EPR）分析研究表明，在白光下可产生活性氧物质，并促进 HSA 的光损伤。
