Opt Express. 2023 Jan 30;31(3):4783-4802. doi: 10.1364/OE.479870.
In this work, authors have developed a portable, sensitive, and quick-response fiber optic sensor that is capable of detection of Aflatoxins B1 (AFB1) quantitatively and qualitatively. Using multi-mode fiber (MMF) and multi-core fiber (MCF), the MMF-MCF-MCF-MMF fiber structure based on symmetric transverse offset splicing and waist-expanded taper is fabricated. The evanescent waves are enhanced to form a strong evanescent field by etching the fiber surface with hydrofluoric acid. To successfully excite the localized surface plasmon resonance phenomenon, gold nanoparticles are deposited on the optical fiber probe's surface. Further, to modify the fiber optic probes, Niobium carbide (NbCT) MXene and AFB1 antibodies are functionalized. NbCT MXene is employed to strengthen the biocompatibility of the sensor and increase the specific surface area of the fiber probe, while AFB1 antibody is used to identify AFB1 micro-biomolecules in a specific manner. The reproducibility, reusability, stability, and selectivity of the proposed fiber probe are tested and validated using various concentration of AFB1 solutions. Finally, the linear range, sensitivity, and limit of detection of the sensing probe are determined as 0 - 1000 nM, 11.7 nm/µM, and 26.41 nM, respectively. The sensor offers an indispensable technique, low-cost solution and portability for AFB1-specific detection in agricultural products and their byproducts with its novel optical fiber structure and superior detecting capability. It is also useful for marine species like fish and consequently affecting health of human body.
在这项工作中，作者开发了一种便携式、灵敏、快速响应的光纤传感器，能够定量和定性检测黄曲霉毒素 B1（AFB1）。使用多模光纤（MMF）和多芯光纤（MCF），基于对称横向偏移拼接和腰扩锥形的 MMF-MCF-MCF-MMF 光纤结构被制造出来。通过用氢氟酸刻蚀光纤表面，增强了消逝波，形成了一个强的消逝场。为了成功激发局域表面等离子体共振现象，在光纤探针的表面沉积了金纳米粒子。此外，为了修饰光纤探针，将碳化铌（NbCT）MXene 和 AFB1 抗体进行功能化。NbCT MXene 被用来增强传感器的生物相容性并增加光纤探针的比表面积，而 AFB1 抗体则被用来以特定的方式识别 AFB1 微生物分子。通过使用不同浓度的 AFB1 溶液对所提出的光纤探针的重现性、可重复性、稳定性和选择性进行了测试和验证。最后，确定了传感探针的线性范围、灵敏度和检测限分别为 0-1000 nM、11.7nm/µM 和 26.41 nM。该传感器提供了一种不可缺少的技术，具有低成本的解决方案和便携性，用于农产品及其副产品中 AFB1 的特异性检测，其新颖的光纤结构和卓越的检测能力。它也适用于鱼类等海洋物种，从而影响人体健康。
