Li Yujie, Luo Binbin, Liu Yanan, Wu Sehngxi, Shi Shenghui, Chen Huiji, Zhao Mingfu
Chongqing Key Laboratory of Optical Fiber Sensor and Photoelectric Detection, Chongqing University of Technology, Chongqing, China.
School of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing, China.
Biomed Opt Express. 2023 Mar 2;14(4):1364-1377. doi: 10.1364/BOE.486717. eCollection 2023 Apr 1.
A label-free microfluidic immunosensor based on the double helix microfiber coupler (DHMC) coated with graphene oxide (GO) was proposed for the specific detection of anti-Müllerian hormone (AMH). Two single-mode optical fibers were twisted in a parallel direction, the coning machine was used to fuse and taper them, and the high-sensitivity DHMC was obtained. To make a stable sensing environment, it was immobilized in a microfluidic chip. And then, the DHMC was modified by GO and bio-functionalized by the AMH monoclonal antibodies (anti-AMH MAbs) for the specific detection of AMH. The experimental results showed that the detection range of the immunosensor for AMH antigen solutions was 200 fg/mL∼50 µg/mL, the detection of limit (LOD) was ∼235.15 fg/mL, and the detection sensitivity and the dissociation coefficient were ∼3.518nm/(log(mg/mL)) and ∼1.85 × 10 M, respectively. The alpha fetoprotein (AFP), des-carboxy prothrombin (DCP), growth stimulation expressed gene 2 (ST2) and AMH serum were used to confirm the excellent specific and clinical properties of the immunosensor, showing that the proposed immunosensor was easy-made and can be potentially applied in the biosensing field.
提出了一种基于涂覆有氧化石墨烯（GO）的双螺旋微光纤耦合器（DHMC）的无标记微流控免疫传感器，用于特异性检测抗苗勒管激素（AMH）。将两根单模光纤沿平行方向扭转，使用锥度机对其进行熔接和拉锥，从而获得高灵敏度的DHMC。为了营造稳定的传感环境，将其固定在微流控芯片中。然后，用GO对DHMC进行修饰，并通过AMH单克隆抗体（抗AMH单克隆抗体）进行生物功能化，以特异性检测AMH。实验结果表明，该免疫传感器对AMH抗原溶液的检测范围为200 fg/mL至50 µg/mL，检测限（LOD）约为235.15 fg/mL，检测灵敏度和解离系数分别约为3.518nm/(log(mg/mL))和1.85×10⁻⁶M。使用甲胎蛋白（AFP）、脱羧凝血酶原（DCP）、生长刺激表达基因2（ST2）和AMH血清来确认该免疫传感器具有优异的特异性和临床性能，表明所提出的免疫传感器易于制备，并且有可能应用于生物传感领域。
