College of Agronomy, Liaocheng University, Liaocheng, 252059, China.
Shandong Key Laboratory of Optical Communication Science and Technology, School of Physics Science and Information Technology, Liaocheng University, Liaocheng, 252059, China.
Biosens Bioelectron. 2023 Sep 15;236:115424. doi: 10.1016/j.bios.2023.115424. Epub 2023 May 23.
Alanine aminotransferase (ALT), a type of inactive enzyme largely present in fish liver cells, is essential for the tricarboxylic acid (TCA) cycle. Monitoring ALT activity in the blood/hepatocellular layer has been demonstrated to be a sensitive sign of liver dysfunction and an essential method for determining the health status of fish. This study details the development of a multi-layer material (hybrids of graphene oxide and multi-walled carbon nanotubes (GO/MWCNTs), gold nanoparticles (AuNPs), and glutamate oxidase (GluOx) enzyme) immobilized localized surface plasmon resonance based unique fiber structure biosensor for the quantitative determination of ALT biomolecules at concentrations ranging from 0 to 1000 U/L. For this kind of detection, a novel taper-in-taper with four tapered (TIT4T) structure based on single-mode fiber has been developed. In addition to AuNPs, GO/MWCNTs were immobilized in the probe's sensing region to increase its LSPR efficiency and sensitivity. Synthesis of AuNPs was carried out utilizing the Turkevich method. The selectivity of the sensor is ensured by the effective immobilization of GluOx on the surface treatment. The linearity of sensor is in the range of 0-1000 U/L, whereas the sensitivity, limit of detection, and detection time are individually found at 7.5 p.m./(U/L), 4.84 U/L and 20 min, respectively. After evaluating the prospective applications of the sensors, the sensors' reusability, reproducibility, stability, pH test, and selectivity have all been found to be satisfactory. Proposed fiber optic biosensors have high sensitivity, robustness, reliability, fast detection, no electromagnetic interference, low cost, real-time monitoring, and biocompatible.
丙氨酸氨基转移酶（ALT），一种主要存在于鱼类肝细胞中的无活性酶，是三羧酸（TCA）循环所必需的。监测血液/肝细胞层中的 ALT 活性已被证明是肝功能障碍的敏感指标，也是确定鱼类健康状况的重要方法。本研究详细介绍了一种多层材料（氧化石墨烯和多壁碳纳米管（GO/MWCNTs）、金纳米粒子（AuNPs）和谷氨酸氧化酶（GluOx）酶的混合物）固定化局域表面等离子体共振基于独特光纤结构生物传感器的开发，用于定量测定浓度范围为 0 至 1000 U/L 的 ALT 生物分子。对于这种检测，已经开发了一种基于单模光纤的新型四锥形（TIT4T）结构的锥形光纤。除了 AuNPs 之外，GO/MWCNTs 也被固定在探针的传感区域中，以提高其 LSPR 效率和灵敏度。AuNPs 的合成是利用 Turkevich 方法进行的。通过表面处理有效固定 GluOx 来确保传感器的选择性。传感器的线性范围为 0-1000 U/L，而灵敏度、检测限和检测时间分别为 7.5 pM/（U/L）、4.84 U/L 和 20 分钟。在评估传感器的潜在应用后，发现传感器的可重复使用性、重现性、稳定性、pH 值测试和选择性均令人满意。提出的光纤生物传感器具有灵敏度高、鲁棒性强、可靠性高、检测速度快、无电磁干扰、成本低、实时监测和生物相容性好等优点。
