Mental Health Research Centre, Moscow, Russia.
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Dokl Biochem Biophys. 2022 Dec;507(1):334-339. doi: 10.1134/S160767292234004X. Epub 2023 Feb 14.
The effects of extracts of ten plant species from Russia and five species from Vietnam on the growth and survival of ciliates Tetrahymena pyriformis were studied. T. pyriformis belongs to the subkingdom Protozoa, which also includes pathogens of protozoan infections. Extraction of dried plants was carried out with acidic and alkaline aqueous solutions, as well as with an aqueous ethanol. Various amounts of extracts were added to the ciliate cells, and the number of cells survived after incubation for 1 and 24 h was recorded. We found that our samples of several plants, including wormwood, harmala, and licorice, similarly to those studied earlier, exhibit antiprotozoal activity, which may indicate that the secondary metabolites are the same in plants from different regions. Using the ciliate T. pyriformis as a model organism, the presence of antiprotozoal activity in extracts of lilac, chondrilla, cinquefoil, hop, and elm was shown for the first time.
研究了来自俄罗斯的 10 种植物和来自越南的 5 种植物的提取物对纤毛虫四膜虫生长和存活的影响。四膜虫属于原生动物亚界，其中还包括原生动物感染的病原体。用酸性和碱性水溶液以及含水乙醇提取干燥的植物。将不同量的提取物添加到纤毛虫细胞中，并记录孵育 1 和 24 小时后存活的细胞数。我们发现，我们的几种植物样本，包括艾草、骆驼蓬和甘草，与之前研究的样本相似，表现出抗原生动物活性，这可能表明来自不同地区的植物中的次生代谢物是相同的。首次使用纤毛虫四膜虫作为模型生物，证明了丁香、软骨草、五倍子、啤酒花和榆树的提取物具有抗原生动物活性。
