Zhonghua Jie He He Hu Xi Za Zhi. 2023 Apr 12;46(4):322-335. doi: 10.3760/cma.j.cn112147-20220701-00553.
Metagenomic next-generation sequencing (mNGS) enables sensitive and unbiased detection of all potential pathogens in lower respiratory tract infection(LRTI). But the lack of consensus and uniformity of applicable clinical scenarios and interpretation of results for mNGS largely limit its utilization. To solve these issues, an expert panel including pulmonary physicians, intensivists and microbiologists is organized by Chinese Thoracic Society. The panel conducted a systematic review of progress and challenges regarding the application of mNGS on etiological diagnosis of LRTI. The panel formulated 17 specific recommendations spanning questions of suitable clinical situations, sample quality control, process of reports interpretation and subsequent medical decisions. Hopefully, this consensus can provide practical guidelines for clinicians and promote the rational application of mNGS in LRTI.
宏基因组下一代测序（mNGS）能够灵敏且无偏倚地检测下呼吸道感染（LRTI）中的所有潜在病原体。但mNGS适用的临床场景以及结果解读缺乏共识和统一标准，这在很大程度上限制了其应用。为解决这些问题，中国胸科学会组织了一个由肺科医生、重症医学专家和微生物学家组成的专家小组。该小组对mNGS在LRTI病因诊断应用方面的进展与挑战进行了系统综述。该小组制定了17条具体建议，涵盖了合适的临床情况、样本质量控制、报告解读流程以及后续医疗决策等问题。希望这一共识能够为临床医生提供实用指南，并促进mNGS在LRTI中的合理应用。
