Huang Qiwei, Wu Tingbin, Guo Yongshi, Wang Lihuan, Yu Xi, Zhu Bo, Fan Longfei, Xin John H, Yu Hui
Guangdong-Hong Kong Joint Laboratory for New Textile Materials, School of Textile Materials and Engineering, Wuyi University, Jiangmen, 529020, China.
Department of Heapatobiliary Surgery, Jiangmen Central Hospital, Jiangmen, 529020, China.
Int J Biol Macromol. 2023 Apr 15;234:123722. doi: 10.1016/j.ijbiomac.2023.123722. Epub 2023 Feb 17.
The ability of autologous platelet-rich plasma (PRP) gel to promote rapid wound healing without immunological rejection has opened new avenues for the treatment of diabetic foot wounds. However, PRP gel still suffers from the quick release of growth factors (GFs) and requires frequent administration, thus resulting in decreased wound healing efficiency, higher cost as well as greater pain and suffering for the patients. In this study, the flow-assisted dynamic physical cross-linked coaxial microfluidic three-dimensional (3D) bio-printing technology, combined with the calcium ion chemical dual cross-linking method was developed to design PRP-loaded bioactive multi-layer shell-core fibrous hydrogels. The prepared hydrogels exhibited outstanding water absorption-retention capacity, good biocompatibility as well as a broad-spectrum antibacterial effect. Compared with clinical PRP gel, these bioactive fibrous hydrogels displayed a sustained release of GFs, reducing the administration frequency by 33 % availably during the wound treatment, but more prominent therapeutic effects such as effective reduced inflammation, in addition to promoting the growth of granulation tissue and angiogenesis, the formation of high-density hair follicles, and the generation of regular ordered and high-density collagen fiber network, which suggested great promise as exceptional candidates for treatment of diabetic foot ulcer in clinical settings.
富含自体血小板血浆（PRP）凝胶促进伤口快速愈合且无免疫排斥反应的能力，为糖尿病足伤口的治疗开辟了新途径。然而，PRP凝胶仍存在生长因子（GFs）快速释放的问题，需要频繁给药，从而导致伤口愈合效率降低、成本增加以及患者更多的痛苦。在本研究中，开发了流动辅助动态物理交联同轴微流控三维（3D）生物打印技术，并结合钙离子化学双重交联方法，以设计负载PRP的生物活性多层壳核纤维水凝胶。制备的水凝胶表现出优异的吸水保水能力、良好的生物相容性以及广谱抗菌作用。与临床PRP凝胶相比，这些生物活性纤维水凝胶显示出GFs的持续释放，在伤口治疗期间有效减少了33%的给药频率，而且具有更显著的治疗效果，如有效减轻炎症，此外还能促进肉芽组织生长和血管生成、形成高密度毛囊以及生成规则有序且高密度的胶原纤维网络，这表明其在临床环境中作为治疗糖尿病足溃疡的极佳候选材料具有很大的前景。
