Opt Lett. 2023 Feb 15;48(4):876-879. doi: 10.1364/OL.478674.
We report on a single capture approach for simultaneous incoherent bright field (BF) and laser-based quantitative phase imaging (QPI). Common-path digital holographic microscopy (DHM) is implemented in parallel with BF imaging within the optical path of a commercial optical microscope to achieve spatially multiplexed recording of white light images and digital off-axis holograms, which are subsequently numerically demultiplexed. The performance of the proposed multimodal concept is firstly determined by investigations on microspheres. Then, the application for label-free dual-mode QPI and BF imaging of living pancreatic tumor cells is demonstrated.
我们报告了一种用于同时进行非相干明场 (BF) 和基于激光的定量相位成像 (QPI) 的单次捕获方法。共路数字全息显微镜 (DHM) 与商用光学显微镜光路中的 BF 成像并行实现，以实现白光图像和数字离轴全息图的空间多路记录，随后对其进行数字解复用。该多模态概念的性能首先通过对微球的研究来确定。然后，展示了用于活胰腺肿瘤细胞无标记双模 QPI 和 BF 成像的应用。
