Wang Jiaxin, Zhang Chiqian, Li Ping, Xu Hui, Wang Weiwu, Yin Weizhao, Wu Jinhua, Hu Zhiqiang
School of Environment and Energy, Guangdong Provincial Key Laboratory of Solid Wastes Pollution Control and Recycling, South China University of Technology, Guangzhou 510006, China.
Department of Civil and Environmental Engineering, Southern University and A&M College, Baton Rouge, LA 70813, USA.
Bioresour Technol. 2023 Apr;373:128744. doi: 10.1016/j.biortech.2023.128744. Epub 2023 Feb 13.
Tetrasphaera-enhanced biological phosphorus removal (T-EBPR) was developed by augmenting conventional EBPR (C-EBPR) with Tetrasphaera to improve phosphorus removal from anaerobic digestate of swine wastewater. At influent total phosphorus (TP) concentrations of 45-55 mg/L, T-EBPR achieved effluent TP concentration of 4.17 ± 1.02 mg/L, 54 % lower than that in C-EBPR (8.98 ± 0.76 mg/L). The enhanced phosphorous removal was presumably due to the synergistic effect of Candidatus Accumulibacter and Tetrasphaera occupying different ecological niches. Bioaugmentation with Tetrasphaera promoted the polyphosphate accumulation metabolism depending more on the glycolysis pathway, as evidenced by an increase in intracellular storage compounds of glycogen and polyhydroxyalkanoates by 0.87 and 0.34 mmol C/L, respectively. The enhanced intracellular storage capacity was coincidentally linked to the increase in phosphorus release and uptake rates by 1.23 and 1.01 times, respectively. These results suggest bioaugmentation with Tetrasphaera could be an efficient way for improved phosphorus removal from high-strength wastewater.
通过用四球藻强化传统的强化生物除磷（C-EBPR）工艺，开发出了四球藻强化生物除磷（T-EBPR）工艺，以提高从猪废水厌氧消化液中去除磷的效果。在进水总磷（TP）浓度为45-55mg/L时，T-EBPR工艺的出水TP浓度为4.17±1.02mg/L，比C-EBPR工艺（8.98±0.76mg/L）低54%。除磷效果的提高可能归因于“候选聚磷菌属”和四球藻占据不同生态位的协同作用。用四球藻进行生物强化促进了聚磷酸盐积累代谢，且更多地依赖糖酵解途径，糖原和聚羟基链烷酸酯的细胞内储存化合物分别增加了0.87和0.34mmol C/L，这证明了这一点。细胞内储存能力的增强分别与磷释放和吸收速率提高1.23倍和1.01倍同时发生。这些结果表明，用四球藻进行生物强化可能是一种从高浓度废水中提高除磷效果的有效方法。
