National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Department of Environmental Engineering, Beijing University of Technology, Beijing 100124, PR China.
National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Department of Environmental Engineering, Beijing University of Technology, Beijing 100124, PR China.
Sci Total Environ. 2022 Oct 10;842:156913. doi: 10.1016/j.scitotenv.2022.156913. Epub 2022 Jun 23.
Tetrasphaera are polyphosphate accumulating organisms (PAOs) that play an important role in enhanced biological phosphorus removal (EBPR) from wastewater. The effect of a wide range of temperature changes (1-30 °C) on phosphorus removal, metabolism and clade-level community structure of Tetrasphaera-dominated PAOs was investigated. At 10 °C, the bioactivities of Tetrasphaera-dominated communities were obviously inhibited and the EBPR efficiency was only 73 %. Yet at 20-30 °C, EBPR efficiency reached 99 % and the relative abundance of Tetrasphaera was up to 90 %. The temperature variation changed the community distribution of Tetrasphaera clades, which was possibly a main reason for EBPR performance. Amino acids and PHA with different contents were intracellular metabolite of Tetrasphaera-dominated communities during phosphorus release and uptake at different temperatures. Moreover, Tetrasphaera fermented protein and amino acids and released VFAs. The outcomes suggested the great potential of Tetrasphaera-PAOs in the treatment of wastewater with varying temperatures and limited carbon sources.
聚磷菌是聚磷酸盐积累菌（PAOs），在强化生物除磷（EBPR）中起着重要作用。本研究考察了温度变化（1-30°C）对聚磷菌为主的 Tetrasphaera 聚磷菌的磷去除、代谢和类群水平群落结构的影响。在 10°C 时，Tetrasphaera 主导的群落生物活性明显受到抑制，EBPR 效率仅为 73%。然而，在 20-30°C 时，EBPR 效率达到 99%，Tetrasphaera 的相对丰度高达 90%。温度变化改变了 Tetrasphaera 菌群的分布，这可能是 EBPR 性能的主要原因。在不同温度下释放和吸收磷时，Tetrasphaera 主导的群落中存在着不同含量的氨基酸和 PHA 等细胞内代谢物。此外，Tetrasphaera 发酵蛋白质和氨基酸并释放 VFAs。结果表明，在处理温度和有限碳源变化的废水中，Tetrasphaera-PAOs 具有巨大的潜力。
