富含 Tetrasphaera 相关聚磷菌的强化生物除磷系统中的微生物组组装机制和功能潜力。
Microbiome assembly mechanism and functional potential in enhanced biological phosphorus removal system enriched with Tetrasphaera-related polyphosphate accumulating organisms.
机构信息
Environmental Science and Engineering Department, Zhejiang University, Hangzhou, 310012, Zhejiang Province, China; Key Laboratory of Coastal Environment and Resources of Zhejiang Province, School of Engineering, Westlake University, Hangzhou, 310030, Zhejiang Province, China; Center of Synthetic Biology and Integrated Bioengineering, Westlake University, Hangzhou, 310030, Zhejiang Province, China.
Key Laboratory of Coastal Environment and Resources of Zhejiang Province, School of Engineering, Westlake University, Hangzhou, 310030, Zhejiang Province, China; Center of Synthetic Biology and Integrated Bioengineering, Westlake University, Hangzhou, 310030, Zhejiang Province, China.
出版信息
Environ Res. 2023 Sep 15;233:116494. doi: 10.1016/j.envres.2023.116494. Epub 2023 Jun 23.
Tetrasphaera-related polyphosphate accumulating organisms (PAOs) are the key functional guilds for enhanced biological phosphorus removal (EBPR) systems. Their biomass enrichment can be enhanced by the nitrification inhibitor allylthiourea (ATU). However, the underlying assembly mechanism and the functional potential of the EBPR microbiome regulated by ATU are unclear. This study investigates the effect of ATU on microbiome assembly and functional potential by closely following the microbiota dynamics in an EBPR system enriched with Tetrasphaera-related PAOs for 288-days before, during and after ATU addition. The results showed that ATU addition increased microbiota structural similarity and compositional convergence, and enhanced determinism in the assembly of EBPR microbiome. During exposure to ATU, Tetrasphaera-related PAOs were governed by homogeneous selection and the dominant species revealed by 16S rRNA gene-based phylogenetic analysis shifted from clade III to clade I. Meanwhile, ATU supply promoted significant enrichment of functional genes involved in phosphate transport (pit) and polyphosphate synthesis and degradation (ppk1 and ppk2), whereas both Nitrosomonas and ammonia monooxygenase-encoding genes (amoA/B/C) assignable to this group of nitrifying bacteria decreased. Moreover, ATU addition relieved the significant abundance correlation between filamentous bacteria Ca. Promineofilum and denitrifying Brevundimonas (FDR-adjusted P < 0.01), damaging their potential synergic or cooperative interactions, thus weakening their competitiveness against Tetrasphaera-related PAOs. Notably, ATU withdrawn created opportunistic conditions for the unexpected explosive growth and predominance of Thiothrix filaments, leading to a serious bulking event. Our study provides new insights into the microbial ecology of Tetrasphaera-related PAOs in EBPR system, which could guide the establishment of an efficient microbiota for EBPR.
聚磷菌（PAOs）是强化生物除磷（EBPR）系统的关键功能菌群。硝化抑制剂烯丙基硫脲（ATU）可以促进其生物量的富集。然而，ATU 调控的 EBPR 微生物组的组装机制和功能潜力尚不清楚。本研究通过密切跟踪富含 Tetrasphaera 相关 PAOs 的 EBPR 系统中微生物群动态，在添加 ATU 之前、期间和之后的 288 天内，研究了 ATU 对微生物组组装和功能潜力的影响。结果表明，ATU 增加了微生物群落结构相似性和组成收敛性，并增强了 EBPR 微生物组组装的确定性。在暴露于 ATU 期间，Tetrasphaera 相关 PAOs 受到同型选择的控制，基于 16S rRNA 基因系统发育分析的优势种从第 III 组转变为第 I 组。同时，ATU 供应促进了参与磷转运（pit）和聚磷酸盐合成和降解（ppk1 和 ppk2）的功能基因的显著富集，而属于这组硝化细菌的亚硝酸盐单加氧酶编码基因（amoA/B/C）则减少。此外，ATU 添加缓解了丝状细菌 Ca. Promineofilum 和反硝化 Brevundimonas 之间显著的丰度相关性（FDR 调整后 P<0.01），破坏了它们潜在的协同或合作相互作用，从而削弱了它们对 Tetrasphaera 相关 PAOs 的竞争力。值得注意的是，ATU 撤出为 Thiothrix 丝状菌的意外爆炸式生长和优势创造了机会条件，导致严重的膨胀事件。本研究为 EBPR 系统中 Tetrasphaera 相关 PAOs 的微生物生态学提供了新的见解，可为 EBPR 建立有效的微生物群提供指导。
Zotero 插件