开发一种用于检测禽类传染病相关病原体的单管实时 PCR 检测系统。
Development of a one-run real-time PCR detection system for pathogens associated with poultry infectious diseases.
机构信息
Center for Infectious Disease Epidemiology and Prevention Research, Tokyo University of Agriculture and Technology, Tokyo, Japan.
Cooperative Division of Veterinary Sciences, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan.
出版信息
J Vet Med Sci. 2023 Mar 30;85(4):407-411. doi: 10.1292/jvms.22-0482. Epub 2023 Mar 16.
Infectious diseases are an important issue in the poultry industry, requiring early diagnosis and countermeasures. To address this, we present a system based on TaqMan real-time PCR to detect pathogen genome in specimens collected from chickens. We designed 12 primer-probe sets for pathogens causing respiratory or systemic symptoms. In field samples, we detected three viruses, including DNA and RNA viruses, and three bacteria. The chicken anemia virus and Avibacterium paragallinarum were detected only in young and laying hens, respectively. Bacteria were detected only in throat swabs, and gallid alphaherpesvirus 2 was detected in different specimens at each developmental stage. Our novel TaqMan real-time PCR system effectively detects pathogen's gene in chickens, while taking age into account.
传染病是家禽业的一个重要问题，需要早期诊断和对策。为此，我们提出了一种基于 TaqMan 实时 PCR 的系统，用于检测从鸡采集的标本中的病原体基因组。我们设计了 12 个针对引起呼吸道或全身症状的病原体的引物-探针组。在现场样本中，我们检测到了三种病毒，包括 DNA 和 RNA 病毒，以及三种细菌。鸡贫血病毒和禽传染性鼻炎支原体仅分别在育雏鸡和产蛋鸡中被检测到。细菌仅在咽喉拭子中被检测到，而禽疱疹病毒 2 则在每个发育阶段的不同标本中被检测到。我们的新型 TaqMan 实时 PCR 系统能够有效地检测鸡中病原体的基因，同时考虑到年龄因素。
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