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大鼠血清和胎牛血清对大鼠淋巴细胞、巨噬细胞及多形核细胞天然杀瘤活性测定的比较效应

Comparative effect of rat and fetal calf serum on measurement of the natural tumoricidal activity of rat lymphocytes, macrophages and polymorphonuclear cells.

作者信息

Reisser D, Fady C, Pelletier H, Lagadec P, Jeannin J F, Olsson N O

机构信息

INSERM U.252, Faculty of Medicine, Dijon, France.

出版信息

Cancer Immunol Immunother. 1989;28(1):34-6. doi: 10.1007/BF00205798.

Abstract

The effect of rat serum versus fetal calf serum on the in vitro natural cytolytic activity of rat lymphocytes, macrophages and polymorphonuclear cells against syngeneic tumour cells was compared. The cytolysis level mediated by the three varieties of effector cells was lower when rat serum was used instead of fetal calf serum to supplement the culture medium. This could explain in part the discrepancies found between in vitro and in vivo studies.

摘要

比较了大鼠血清与胎牛血清对大鼠淋巴细胞、巨噬细胞和多形核细胞针对同基因肿瘤细胞的体外自然细胞溶解活性的影响。当使用大鼠血清而非胎牛血清补充培养基时,三种效应细胞介导的细胞溶解水平较低。这可以部分解释体外研究与体内研究之间发现的差异。

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