由二甲基肼诱导的大鼠结肠腺癌的致瘤性和非致瘤性细胞变体中的活化K-ras。
Activated K-ras in tumorigenic and non-tumorigenic cell variants from a rat colon adenocarcinoma, induced by dimethylhydrazine.
作者信息
Caignard A, Kitagawa Y, Sato S, Nagao M
机构信息
Biochemistry Division, National Cancer Center Research Institute, Tokyo.
出版信息
Jpn J Cancer Res. 1988 Feb;79(2):244-9. doi: 10.1111/j.1349-7006.1988.tb01583.x.
Using NIH3T3 cell transfection assay, activated c-K-ras was detected in two cell lines, TRb and TSb, obtained from a single colon adenocarcinoma induced in a rat by 1,2-dimethylhydrazine. TRb cells give rise to progressive and metastatic tumors in the syngeneic rats, whereas TSb cells only induce regressive tumors. Levels of K-ras transcripts in TRb and TSb cells were higher than that of NIH3T3 cells, but no difference was found between TRb and TSb cells. No significant difference was observed in expression levels of c-myc in these two cell lines. c-fos expression was, however, significantly lower in TRb than TSb cells.
采用NIH3T3细胞转染试验，在从用1,2 - 二甲基肼诱导的大鼠单个结肠腺癌中获得的两个细胞系TRb和TSb中检测到激活的c-K-ras。TRb细胞在同基因大鼠中产生进行性和转移性肿瘤，而TSb细胞仅诱导退行性肿瘤。TRb和TSb细胞中K-ras转录本水平高于NIH3T3细胞，但TRb和TSb细胞之间未发现差异。在这两个细胞系中，c-myc的表达水平未观察到显著差异。然而，TRb细胞中c-fos的表达明显低于TSb细胞。
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