Key Laboratory of Molecular Biology for Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, 400016, People's Republic of China.
The First Clinical College, Chongqing Medical University, Chongqing, 400016, People's Republic of China.
Med Oncol. 2023 Feb 15;40(3):96. doi: 10.1007/s12032-023-01966-2.
Tubulin γ-1 (TUBG1) is a highly conserved component of the centrosome and its deregulation is involved in the development of several types of cancer. However, the role of TUBG1 in hepatocellular carcinoma (HCC) remains unclear. In this study, we found that TUBG1 was upregulated in human HCC cells and tissues and that TUBG1 upregulation was associated with promoter hypomethylation in HCC tissues. TUBG1 knockdown suppressed the proliferation, invasion, and migration of HCC cells. While TUBG1 expression was positively correlated with CD4 + memory T lymphocyte infiltration, it was negatively correlated with CD4 + regulatory T-cell infiltration in human HCC tissues. Furthermore, TUBG1 expression was positively correlated with the expression of genes involved in cell division. Noticeably, high expression of TUBG1 was associated with poor prognosis in patients with HCC. Overall, our findings revealed that TUBG1 promotes hepatocarcinogenesis by increasing proliferation, invasion, and migration of HCC cells and may regulate T lymphocyte infiltration. The current findings provide important insights into TUBG1 regulation in HCC, which could provide new therapeutic targets for hepatocarcinoma which has a very high incidence and mortality rate worldwide.
微管蛋白 γ-1（TUBG1）是中心体的高度保守组成部分，其失调与几种类型的癌症的发展有关。然而，TUBG1 在肝细胞癌（HCC）中的作用尚不清楚。在这项研究中，我们发现 TUBG1 在人 HCC 细胞和组织中上调，并且 TUBG1 上调与 HCC 组织中的启动子低甲基化有关。TUBG1 敲低抑制 HCC 细胞的增殖、侵袭和迁移。虽然 TUBG1 表达与 CD4+记忆 T 淋巴细胞浸润呈正相关，但与 CD4+调节性 T 细胞浸润呈负相关。此外，TUBG1 表达与参与细胞分裂的基因表达呈正相关。值得注意的是，TUBG1 的高表达与 HCC 患者的预后不良相关。总体而言，我们的研究结果表明，TUBG1 通过增加 HCC 细胞的增殖、侵袭和迁移促进肝癌发生，并且可能调节 T 淋巴细胞浸润。这些发现为 HCC 中 TUBG1 的调控提供了重要的见解，这可能为全世界发病率和死亡率都很高的肝癌提供新的治疗靶点。
